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5 Salmonella Water Malaysia

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  • 1. EFFECT OF INDOOR ENVIRONMENT ON THE SURVIVAL OF SALMONELLA ENTERITIDIS AND SALMONELLA TYPHIMURIUM
    K. Ganapathy & Kok H. S.
    Faculty of Veterinary Medicine
    University Putra Malaysia
  • 2. INTRODUCTION
    • Protect health and safety of human.
    • Protect export market & global acceptance.
    • Protect health, production and welfare of chickens.
    • Salmonella spp infection in chickens
    • Prevention/control and eradication of Salmonellae infection - important
  • 3. CONTROL & PREVENTION
    Antibiotics
    Non-absorbable chemicals
    Acidifiers
    Prebiotics
    Probiotics
    Phytogenicadditives
    Antibodies
    Other biological substances
  • 4. PROBLEM STATEMENT
    • Numerous acidifiers brands for water treatment
    • Comparative efficacy studies – no ‘standard’ method
    • Efficacy of acidifiers under tropical climate is unknown
    • Limitation of in-vivo studies (cost, facilities, repeatability and etc)
  • 5. OBJECTIVE
    • To determine survival of SE and ST in the drinking water under Malaysian indoor environment.
    • 6. To determine survival of SE and ST in of 7 different products in water under Malaysian indoor environment.
  • MATERIAL & METHODOLOGY
     Salmonella enteritidis (SE) (Salmonella entericaserovarenteritidis)
     Salmonella typhimurium (ST) (Salmonella entericaserovartyphimurium)
  • 7. IN-WATER TREATMENT
  • 8. Items
    Homogenized
    1ml overnight culture
    9ml
    500ml
    Autoclaved tap water
    10ml mixture
    2hr
    4hr
    6hr
    12hr
    24hr
    100ul
    Experiment 1 : In-water Treatment
  • 9. 10-fold dilutions
    900ul deionized distilled water
    Vortex mixing
    100ul
    plating
    20ul
    24hours
    incubation 37OC
    counting
  • 10. Results: SE survival in Sterile - Water
  • 11. NOTE
    Out of 7 acidifiers and/or prebiotics tested at the dosage recommended by manufactures only two products worked and had effect against the Salmonella enteritidis (SE).
    The acidifiers were made from different mixtures of acids such as formic, lactic, propionic, acetic, citric,. The only acidifier that worked, the problem was that it reduced so much pH of water that birds will not drink it.
  • 12. Acidifier-treated water
  • 13. Items
    Homogenized
    1ml overnight culture
    9ml
    500ml
    Autoclaved tap water
    10ml mixture
    2hr
    4hr
    6hr
    12hr
    24hr
    100ul
    Experiment 1 : In-water Treatment
  • 14. 10-fold dilutions
    900ul deionized distilled water
    Vortex mixing
    100ul
    plating
    20ul
    24hours
    incubation 37OC
    counting
  • 15. Results: SE survival in acidified-water
  • 16. In-water Treatment ST
  • 17. Results: ST survival in water
  • 18. NOTE
    Out of 7 acidifiers and/or prebiotics tested at the dosage recommended by manufactures only two products worked and had effect against the Salmonella typhimurium (ST).
    The acidifiers were made from different mixtures of acids such as formic, lactic, propionic, acetic, citric,. The only acidifier that worked, the problem was that it reduced so much pH of water that birds will not drink it.
  • 19. Acidifier-treated water
  • 20. Results: ST survival in acidified water
  • 21. Discussion
    • Current methodology
    • 22. High survival
    • 23. Drinking water
    • 24. Indoor environment
    • 25. Suitable for efficacy studies…
    • 26. Digester Broiler showed remarkable
    reduction of SE and ST
  • 27. Conclusion
    In-water - a workable in vitro
    methodology was found.
    - Digester Broiler Liquid
    Dosage 0.5ml / 1L water
    Reduced significantly SE & ST titers.
  • 28. Ultimate assessment…
    In-water
    ….in vivo studies
    under control (experimental)
    and/or field condition
  • 29. Acknowledgement
    Research students & assistants
    - Dr.Mahadevan
    - Mr. Phi Lip
    - Mr. Tan Chin Giap
    Prof. Ho & Ms.Vicky (IBS)
    Prof MadyaDr.Saleha, En.Kamar, En.Hajar
    Pharmaceutical companies including Rhonema (M) Sdn. Bhd.
    Other directly or indirectly assisted…