Estimating wheat root biomass
using ground penetrating radar
Sean M. Thompson, Alfredo Delgado, R. Esten Mason,
C. Mariano...
Phenotyping roots
0-30 cm

30-60 cm

60-90 cm

90-120 cm

2

© Sean M. Thompson 2013 - All Rights Reserved
Hypothesis
GPR is capable of phenotyping root biomass of wheat in
situ.

Objectives
Develop the use of GPR for phenotyping...
Two-way travel time (nanoseconds)

Antenna	
  

Antenna	
  

00	
  –	
  
	
  
	
  
02	
  –	
  
	
  
	
  
04	
  –	
  
	
  
...
0-30 cm

Plot width

30-60 cm

Antenna	
  

Antenna	
  

Antenna	
  

60-90 cm

90-120 cm

5

© Sean M. Thompson 2013 - Al...
Waterlogging treatment

Control treatment
March 20, 2013 Pre-Waterlogging

April 8, 2013 (19) Waterlogging

April 16, 2013...
Geophysical
Archaeometry
Laboratory

GPR-Slice
7

© Sean M. Thompson 2013 - All Rights Reserved
Spatial analysis

8

© Sean M. Thompson 2013 - All Rights Reserved
Time slices

9

© Sean M. Thompson 2013 - All Rights Reserved
Conclusions
•  Spatial relationship between
plots and GPR amplitude
return in both control and
treatment.
•  In field spat...
Looking forward
•  CIMMYT
–  Deeply rooted partnership

•  IDS Multi Array System
–  Root Depth / Biomass
–  Root Architec...
isdcorporation.com
12

© Sean M. Thompson 2013 - All Rights Reserved
Support
Texas A&M AgriLife Research
Texas Cropping System Grant
Texas A&M University, Soil & Crop Sciences

Texas Wheat Pr...
Team members
Texas A&M AgriLife
•  Wheat Physiology, Small Grains Breeding
•  Small Grains Extension

University of Arkans...
Sean M. Thompson
sean.thompson@tamu.edu | precision-phenotyping.com

Alfredo Delgado
adelgado.tamu@gmail.com| soilcrop.tam...
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Estimating wheat root biomass using ground penetrating radar

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Remote sensing –Beyond images
Mexico 14-15 December 2013

The workshop was organized by CIMMYT Global Conservation Agriculture Program (GCAP) and funded by the Bill & Melinda Gates Foundation (BMGF), the Mexican Secretariat of Agriculture, Livestock, Rural Development, Fisheries and Food (SAGARPA), the International Maize and Wheat Improvement Center (CIMMYT), CGIAR Research Program on Maize, the Cereal System Initiative for South Asia (CSISA) and the Sustainable Modernization of the Traditional Agriculture (MasAgro)

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Estimating wheat root biomass using ground penetrating radar

  1. 1. Estimating wheat root biomass using ground penetrating radar Sean M. Thompson, Alfredo Delgado, R. Esten Mason, C. Mariano Cossani, Matthew P. Reynolds, Amir M. H. Ibrahim, Ronald E. Lacey, Robert A. Washington-Allen, and Dirk B. Hays CIMMYT Remote Sensing - Beyond Images December 14-15, 2013 © Sean M. Thompson 2013 - All Rights Reserved
  2. 2. Phenotyping roots 0-30 cm 30-60 cm 60-90 cm 90-120 cm 2 © Sean M. Thompson 2013 - All Rights Reserved
  3. 3. Hypothesis GPR is capable of phenotyping root biomass of wheat in situ. Objectives Develop the use of GPR for phenotyping below ground biomass, in the context of higher yield stability during drought and high temperature stress. •  The validation of GPR to non-destructively detect wheat roots in a controlled and stressed environment for correlation to traditional destructive root measurements. 3 © Sean M. Thompson 2013 - All Rights Reserved
  4. 4. Two-way travel time (nanoseconds) Antenna   Antenna   00  –       02  –       04  –       06  –       08  –       10  –       12  –     Soil Sci. Soc. Am. J. 67:1607–1615 (2003) 4 © Sean M. Thompson 2013 - All Rights Reserved
  5. 5. 0-30 cm Plot width 30-60 cm Antenna   Antenna   Antenna   60-90 cm 90-120 cm 5 © Sean M. Thompson 2013 - All Rights Reserved
  6. 6. Waterlogging treatment Control treatment March 20, 2013 Pre-Waterlogging April 8, 2013 (19) Waterlogging April 16, 2013 (27) Waterlogging (Images: R. Esten Mason) 6 © Sean M. Thompson 2013 - All Rights Reserved
  7. 7. Geophysical Archaeometry Laboratory GPR-Slice 7 © Sean M. Thompson 2013 - All Rights Reserved
  8. 8. Spatial analysis 8 © Sean M. Thompson 2013 - All Rights Reserved
  9. 9. Time slices 9 © Sean M. Thompson 2013 - All Rights Reserved
  10. 10. Conclusions •  Spatial relationship between plots and GPR amplitude return in both control and treatment. •  In field spatial variability will need to be considered in future GPR studies. •  Root biomass and depth can be visualized by GPR . 10 © Sean M. Thompson 2013 - All Rights Reserved
  11. 11. Looking forward •  CIMMYT –  Deeply rooted partnership •  IDS Multi Array System –  Root Depth / Biomass –  Root Architecture –  Field-Based HTP •  GPR-Slice –  Rapid Data Analysis –  User Friendly Interface –  In Field Data Processing isdcorporation.com 11 © Sean M. Thompson 2013 - All Rights Reserved
  12. 12. isdcorporation.com 12 © Sean M. Thompson 2013 - All Rights Reserved
  13. 13. Support Texas A&M AgriLife Research Texas Cropping System Grant Texas A&M University, Soil & Crop Sciences Texas Wheat Producers Board Wheat Physiological Breeding Texas A&M University, Small Grains Breeding Program International Maize and Wheat Improvement Center Global Wheat Program, Wheat Physiology Consultative Group on International Agricultural Research United States Department of Agriculture U.S. Borlaug Fellows in Global Food Security Purdue University, Center for Global Food Security 13 © Sean M. Thompson 2013 - All Rights Reserved
  14. 14. Team members Texas A&M AgriLife •  Wheat Physiology, Small Grains Breeding •  Small Grains Extension University of Arkansas •  Small Grains Breeding CIMMYT •  Physiology •  Breeding •  Biostatistics 14 © Sean M. Thompson 2013 - All Rights Reserved
  15. 15. Sean M. Thompson sean.thompson@tamu.edu | precision-phenotyping.com Alfredo Delgado adelgado.tamu@gmail.com| soilcrop.tamu.edu “Do not only practice your art, but force your way into secrets, for it and knowledge can raise man to the divine.” -Beethoven 15 © Sean M. Thompson 2013 - All Rights Reserved
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