Genetic diversity of cassava landraces in the great lakes region of Africa assessed using SSR markers

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  • 1. Genetic diversity of cassava landraces in the great lakes region of Africa assessed using SSR markers A. Pariyo1, 2*, Y. Baguma2, R. Kawuki2, C. Omongo2 , T. Alicai2, R. Edema1, G. Gashaka4, H. Obiero3, M, Fregene6, I. Njuki5, M. Ferguson5 and A, Bua2 1MakerereUniversity Kampala (MAK), Kampala, Uganda; 2National Crops Resources Research Institute (NaCRRI), Kampala Uganda; 3Kenya Agricultural Research Institute (KARI), Kakamega, Kenya; 4Institut des Sciences Agronomiques du Rwanda (ISAR), Rwanda; 5International Institute of Tropical Agriculture (IITA), Nairobi, Kenya; 6International Centre for Tropical Agriculture (CIAT), Cali, Colombia *e-mail: apariyo@agric.mak.ac.ug, tkakau@yahoo.co.uk Background Results Assessment of genetic diversity using molecular tools has Farmers preference based on plant archtecture and biological fitness become a key approach in the repertoire of the plant breeder. The genetic diversity of cassava in the great 50 lakes region of Africa is poorly understood. Landraces 40 from Uganda, Western Kenya and Rwanda were analyzed % reason for 30 choice Y 20 for genetic diversity using SSR markers. Farmers in Rwanda Uganda 10 have the highest 0 Kenya Materials and Methods desire for high Y PR NV EM yielding cultivars AV ST Rwanda 287 farmers interviewed & Data was collected & Y = Yield; PR = Pest resistance; NV = New variety; EM = Early maturing; AV = Availability & ST = Storability 466 cultivars phenotyped analyzed on: Cassava Variety selection by farmers based on culinary qualities • Farmers cassava selection criteria based on: 40 35 1. Plant biological fitness 30 25 2. Culinary qualities % of farmers 20 U 15 • Genomic SSR diversity Majority of Farmers in 10 Kenya select for bread 5 Uganda 0 quality; in Rwanda 1 Kenya 2 3 select for Sweetness & 4 5 6 in Uganda are diverse Traits Rwanda 1 = Sweetness; 2 = Brewing; 3 = Chips, 4 = Bread (Flour); 5 = Vegetable; 6 = Boiling 466 leaf sample collected for SSR analyses 0.01 Cassava cultivars in Rwanda are genetically distinct from Uganda and Capillary sequencer / Kenya Uganda Kenya Rwanda ABI3730 Conclusion and Recommendations There is sufficient genetic diversity of cassava that can be used to enhance breeding and conservation in East African Region. DNA extraction Reference Fregene et al. (2003). Theoretical and applied Genetics. 106(6): 1083-1093 PCR amplicons tested Acknowledgement Ginés Mera Fellowship Fund, Code: C – 055 – 06