Eco-efficiency & Abiotic Stress

1,089 views

Published on

Presentation by Jagadish Rane for the CIAT KSW 2009

Published in: Technology
0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total views
1,089
On SlideShare
0
From Embeds
0
Number of Embeds
3
Actions
Shares
0
Downloads
48
Comments
0
Likes
0
Embeds 0
No embeds

No notes for slide

Eco-efficiency & Abiotic Stress

  1. 1. Eco-efficiency & Abiotic Stress Global food demand Global population Tolerance to Abiotic Stress Crop
  2. 2. Historical Warnings of Future Food Insecurity with Unprecedented Seasonal Heat David. S. Battisti, et al. Science 323 , 240 (2009)
  3. 3. The goal <ul><li>Agronomically superior genotypes with a stack of </li></ul><ul><li>Desirable genes associated with adaptive traits </li></ul><ul><li>Adaptive traits associated with abiotic stress tolerance </li></ul>
  4. 4. Challenges ahead Carrying forward the genome science-progress to that at the whole plant and crop levels in the field. <ul><li>So far: Limited progress </li></ul><ul><ul><li>Complexities in stress-tolerance </li></ul></ul><ul><ul><ul><li>Molecular , Cell , Whole plant level </li></ul></ul></ul><ul><ul><ul><li>G X E </li></ul></ul></ul><ul><li>Gene –Trait association? </li></ul><ul><ul><li>1000 to 50000 plants to be genotyped and phenotyped </li></ul></ul><ul><ul><li>under a particular stress environment </li></ul></ul><ul><li>At present: More hopes </li></ul><ul><ul><li>to solve complexities </li></ul></ul><ul><ul><ul><li>Recent advances in molecular biology </li></ul></ul></ul><ul><ul><ul><li>Robust techniques and tools </li></ul></ul></ul><ul><ul><ul><li>Crop genomes- sequencing at its peak </li></ul></ul></ul>Genomics Genotyping RIL, NIL, CSSL Mutants Transgenic Conventional Breeding   Base material & Product Mapping population Gene/Molecular Markers for MAS
  5. 5. <ul><li>Year 2000 Sanger capillary- based high quality reference: </li></ul><ul><li>AGI = 500 people-7 years @ $70,000,000. per genome </li></ul><ul><li>Perlegen tiling array based: yr. 2006 </li></ul><ul><li>50 people- 1 year @ $70,000 per genome </li></ul><ul><li>Illumina-based: yr 2008 </li></ul><ul><li>2 postdocs - 7 days @ $7,000 per genome @ 50 x coverage </li></ul><ul><li>The future- yr 2014? </li></ul><ul><li>Single molecule-based (e.g. Pacific Bioscience) </li></ul><ul><li>2-3 min @ $70 per genome </li></ul>Evolution of Gene Sequencing Technology Can Phenotyping keep pace with genotyping ?
  6. 6. Initiatives at CIAT needs to be scaled up Boom system for precise irrigation & Network of probes for soil moisture profile <ul><li>Simulation of natural upland rice environment </li></ul><ul><ul><li>& rainout shelter </li></ul></ul>Infrared thermography for remote sensing of stress tolerance
  7. 7. Why a phenotyping platform? <ul><li>To facilitate high throughput phenotyping under field and controlled environment </li></ul><ul><li>Automatic, precise, efficient management of stress </li></ul><ul><li>Precisely characterizing plant responses and growth environment </li></ul><ul><li>With noninvasive tools and methods </li></ul>
  8. 8. Visualized Phenomics at CIAT PDA with barcode reader + User friendly data acquisition Controlled environment and automated phenotyping (http://www.lemnatec.com/)

×