Here are the traces. You can see some FIMS data in the document fields (eg identified by, tissue id). You will also notice a binning column (see the following slide)
Binning is a huge timesaver –high binned reads are OK to pass onto the next step in the workflow, and low binned reads can be rejected for resequencing. This means that only ~10% of the data needs to be examined by the scientist
*Let’s trim the reads to try and move them into higher bins. Run through the options quickly. Geneious annotates the trims, rather than just removing them like other programs. This means you don’t lose any data, and can re-trim later on if you want to redo your workflow
When the process is finished, you get a bunch of pairwise assemblies, which are binned according to the binning parameters (center box). You also get an assembly report telling you exactly which reads were and were not assembled.
I this case we are sequencing a coding region, so as an additional validation step we can color bases by their translaton to look for internal stop codons (they will show up in black)
We can check that simlar taxa are grouped together in the tree (a good indication that the sequences we are getting back match the organisms we think we collected)
Entering Data The checkboxes on the left tell Geneious whether to edit the fields. If checked, all selected reactions will have that field set to the entered value. If unchecked, the fields will be left as they are.