Ralph Imondi - Opening Plenary

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Ralph Imondi - Opening Plenary

  1. 1. Barcoding the Kelp Forests of California’s Channel Islands National ParkRalph Imondi, David Kushner, Daniel Distel, Timery Deboer, Robert Hanner, and Linda Santschi
  2. 2. Caenorhabditis elegans Hirudo medicinalis Schistocerca nitensDrosophila melanogaster Danio rerio Xenopus laevis Gallus domesticus Rattus norvegicus Mus musculus
  3. 3. Energizing science through interdisciplinary studies concepts environmentalearth science science & policy ecology biochemistry molecular cell biology developmental biology biology tools processes computer genetics science
  4. 4. Mending the disconnect betweenscientific research and science educationscientific research community 2˚ educational community
  5. 5. Emphasizing the scientific discovery process exploration & discovery TESTING IDEAS community benefits analysis & & outcomes feedback
  6. 6. Potential student entry points into the DNA barcoding workflow utilizing barcode data application specimen collecting and user tissue sampling research user BOLD GenBank EOL general public
  7. 7. Student engagement in the entire workflowspecimen collecting
  8. 8. tissue processing
  9. 9. creating specimen pages on BOLD
  10. 10. generating COI amplicons
  11. 11. analyzing, editing, and uploading sequence data
  12. 12. California’s northern Channel Islands
  13. 13. The northern Channel Islands are fringed by extensive kelp forest habitat
  14. 14. Upwelling features near the islandscontribute to kelp forest productivity ** * *
  15. 15. San Luis Obispo California Current Oregonian Province Point Conception Santa Barbaracold subartic water Ventura Los Angeles warm equatorial Pacific water California Countercurrent Californian Province
  16. 16. The confluence of major current systemsgives rise to ecologically diverse species assemblagesSan Miguel Santa Rosa Santa Cruz AnacapaPycnopodia helianthoides variableHypsypops zone transition rubicundusboreal special temperate biota assemblage biota
  17. 17. The CINP monitors up to 37 sites around the northern Channel Islands San Miguel Santa Cruz Santa Rosa Anacapa the sites reflect the broad range of conditions and biological assemblages found in the park
  18. 18. The park collects abundance data for select species of algae, invertebrates, and fishesC. pictusO. californica C. gigantea spadicea P. helianthoides miniata C. punctipinnis nicholsiiP. elegansB. stearnsii K. rufescens H. kelletii P. californica M.californicus E. jacksoni S. californicusL. lofotensisU. chilensis M. crenulata H. rubicundus D. undosa M. imbricata L. californica O. dalli
  19. 19. Sebastes barcoding projectS. mystinus S. chrysomelas S. auriculatus S. rosaceusS. dalli S. carnatus S. semicinctus S. serricepsS. umbrosus S. atrovirens S. miniatus S. serranoides
  20. 20. number of species 1-5 6 - 10 11 - 15 16 - 20number21 species of - 25 26 - 30 31 - 35 36 - 40 41 - 45 46 - 50 51 - 55 56 - 60
  21. 21. Rockfishes are a heavily exploited by commercial and recreational fisheries Pacific Ocean Perch Widow 1.0 Yelloweye Bocacciorelative depletion 0.8 Darkblotched Cowcod 0.6 Canary 0.4 0.2 0 1960 1965 1970 1975 1980 1985 1990 1995 2000 2005 2010
  22. 22. The inability to identify larval rockfish hinders efforts to characterize theirdistribution, abundance and life history traits
  23. 23. Forms of student engagementDirect (residential program for students) CMBIndirect (professional development program for teachers) training instructional materials high standardized lab protocols school transportable equipment CMB
  24. 24. Collaboration framework (scientific strand) students CINP resource management & enforcement personnel UCSB Marine Operations Facility volunteers specimencollection & tissue processing sample curation & data accession Ocean distribution Genome CCDB taxonomic Legacy verification & vouchering SBMNH
  25. 25. Economic workforce development linkages Recruitment Equipment support CMB high schoolsResidential program for studentsProfessional development program
  26. 26. Economic workforce development linkages Recruitment North Valley Biotech Center Bay Area Biotech CenterEquipment support LA/OC Biotech Center SoCal Biotech Center
  27. 27. Geographic Distribution of Participants Arcadia Bakersfield Calabasas Camarillo Carpinteria Chatsworth Compton Dana Point Dos Pueblos Dublin Fontana Granada Hills HollywoodHuntington Beach La Jolla Lakewood Long Beach Los Angeles Los Altos Los Gatos 300 km Los Osos Mission Viejo Moorpark North Hollywood Norwalk
  28. 28. Project statusNORTHERN CHANNEL ISLANDS SEBASTES BARCODE PROJECT [CMBS]
  29. 29. Project statusChannel Islands Kelp Forest and MPA Fish and Invertebrates [CNOI]
  30. 30. Geographic Distribution of Participants 300 km
  31. 31. Expanding student access to specimens Klamath Sierra San Nevada Francisco Bay Area Mohave Desert Mediterranean Sonoran Coast Desert
  32. 32. Alignment of student-led barcoding activitieswith NPS I&M activities and youth programs
  33. 33. Technology Resource Development
  34. 34. Technology Resource Development www.DNAbarcodingAssistant.org
  35. 35. Technology Resource Development CMB Guelph meeting the barcode data standard BOLD user user BOLD data portal
  36. 36. Technology Resource Development Guelph meeting the high barcode data schools standard BOLD pre-BOLD professional non-professional user user BOLD professional data portal user
  37. 37. Biotechnology ExplorerTM
  38. 38. An Internet platform to broaden studentengagement in DNA barcoding and iBOL
  39. 39. Acknowledgements Robert HannerDavid Kushner Sujeevan RatnasinghamSonia IbarraEric MooneyJoshua Sprague Eric Hochberg Paul Valentich-Scott Dan Distel Abigail Fusaro Timery Deboer Jeffery O’Neal Milton Love Wendi Johnston Samuel Hammond Christoph Pierre

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