The identification of Larvae, eggs     and stomach contents… three studies of the application  of barcodes on Mexican fish...
Contoy                  Punta Allen                                       Contoy                       B. Chinchorro      ...
There exist many places of reeffish spawning aggregations andnursery areas of economic andecological importance.          ...
What fish species are living in this region?                     Where are they?                           Adults         ...
Applying barcodeswe have found some interesting things…
Barcodes help us to identify the larvae…69 new records for the Mexican Caribbeanonly at larvae stage       Awaous banana ...
766 adult species with their larvae, previously unknown.Family GerreidaeGenus Eucinostomus   E. jonesii               E. ...
Osteology description Eucinostomus jonesii                    clearing technique
Barcode allows us to know the distribution and thetime of the appearance of larvae                                        ...
7Family GerreidaeGenus Eucinostomus   E. jonesii        E. melanopterus         E. gula                                   ...
Egg distribution and the time of the appearance …
Regalecus glesne    King of herrings                                      Cozumel                                         ...
Lachnolaimus maximus       Hogfish                       Xcalak        adults                                1-30 Jan-2007
The case of…Mycteroperca bonaci  Life cycle of the  species             when and where the fishes spawn               deve...
Lionfishsituation    inMexico…
There are several reasons to beconcerned about their presence:They have venomous spines that could            Is the firs...
Stomach contents                   What can I do?                       Use                     Barcode…
Stomach contents analysis                          Cozumel, Xcalak,Contoy island122 Pterois volitans      Banco Chinchorro...
Results          170 sequences were compared with a          reference library of DNA barcodes.      125 sequences matched...
Fish:                     30 different species were identified (12 families)                    5 new records for Mexican...
Tripterygiidae                 Enneanectes altivelis          Enneanectes boehlkei Labrisomidae                           ...
Grammatidae                       Monacanthidae Gramma loreto                     Monacanthus tuckeri                Ptero...
Crustacean45 sequencesUsing the barcodes wecould identify 20 differentspecies.3 Orders:Euphausiacea…..1 speciesStomatopoda...
Euphausiacea Euphausia americana                  Euphausia spStomatopoda                       Pseudosquilla sp.         ...
Decapoda: 17 species    Hyppolytidae         Thor sp.                                             Alpheidae   (CRU120)    ...
In BoldDecapoda: 141 Familiesand 2,049 sp sequencedThe BOLD data base is notcomplete yet….
Conclusions1. This is an example of how important the barcodes are in the support   of sustainable fisheries.2. With the h...
Acknowledgements:NOAA y RSMASCONANP – Xcalak y Banco ChinchorroCONABIO y BARCODE of LIFEECOSURITCH                        ...
Thanks for your  attention!We need to visitthese places….                    Xcalak
Upcoming SlideShare
Loading in...5
×

Martha Valdez-Moreno - Vertebrates Plenary

1,288

Published on

The identification of Larvae, eggs and stomach contents…
three studies of the application of barcodes on Mexican fish.

Published in: Technology, Business
0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total Views
1,288
On Slideshare
0
From Embeds
0
Number of Embeds
0
Actions
Shares
0
Downloads
7
Comments
0
Likes
0
Embeds 0
No embeds

No notes for slide

Martha Valdez-Moreno - Vertebrates Plenary

  1. 1. The identification of Larvae, eggs and stomach contents… three studies of the application of barcodes on Mexican fish. Martha Elena Valdez Moreno Lourdes Vásquez Yeomans Carolina Quintal Lizama Dalia Cázarez Carrillo Elva Leyva
  2. 2. Contoy Punta Allen Contoy B. Chinchorro Xcalak Mahahual Banco ChinchorroTheMexicanCaribbean Xcalak
  3. 3. There exist many places of reeffish spawning aggregations andnursery areas of economic andecological importance. To ensure the sustainability of these areas as integrated ecosystems and the possibility to protect them, it is essential to know…
  4. 4. What fish species are living in this region? Where are they? Adults Juvenile 74 mm LT 22 mm LT Larvae ? 19 mm LT Eggs 7 mm LT
  5. 5. Applying barcodeswe have found some interesting things…
  6. 6. Barcodes help us to identify the larvae…69 new records for the Mexican Caribbeanonly at larvae stage Awaous banana Pterois volitans* River goby Lionfish Cozumel
  7. 7. 766 adult species with their larvae, previously unknown.Family GerreidaeGenus Eucinostomus E. jonesii E. melanopterus E. gula Using barcode The external morphology is not useful to identify these larvae…..
  8. 8. Osteology description Eucinostomus jonesii clearing technique
  9. 9. Barcode allows us to know the distribution and thetime of the appearance of larvae species economically important 8-29/march/06 19/march/07 Mycteroperca bonaci Black grouper Aug/2005 Thunnus atlanticus Mar/2006 Blackfin tuna
  10. 10. 7Family GerreidaeGenus Eucinostomus E. jonesii E. melanopterus E. gula Jan-AugAll year Mar-may Xcalak
  11. 11. Egg distribution and the time of the appearance …
  12. 12. Regalecus glesne King of herrings Cozumel 9-Apr-2009 this fish could approach the coast in the north ofthe largest teleost fish of the world (8-10 m) the Mexican Caribbean for spawning or feeding
  13. 13. Lachnolaimus maximus Hogfish Xcalak adults 1-30 Jan-2007
  14. 14. The case of…Mycteroperca bonaci Life cycle of the species when and where the fishes spawn development and maintenance fisheries propose protected areas
  15. 15. Lionfishsituation inMexico…
  16. 16. There are several reasons to beconcerned about their presence:They have venomous spines that could Is the first non-injure divers. native marine fish established in the Western North Atlantic andMexico has many places that are Caribbean Sea.world class scuba diving destinationLionfish are predators that consume“mexican” native reef species!!! What kind of prey do they eat in the mexican caribbean ?
  17. 17. Stomach contents What can I do? Use Barcode…
  18. 18. Stomach contents analysis Cozumel, Xcalak,Contoy island122 Pterois volitans Banco Chinchorro,Puerto Morelosfixed in alcohol (2009) Playa del Carmen 35 fresh digestive tracts were Mahahual placed in ethylic alcohol 96% Xcalak (2010) 90% showed a high degree of digestion of the tissue A total of 157 stomachs were examined only 144 had stomach contents We separated 330 prey items
  19. 19. Results 170 sequences were compared with a reference library of DNA barcodes. 125 sequences matched with fishes 45 sequences matched with crustacean
  20. 20. Fish: 30 different species were identified (12 families)  5 new records for Mexican Caribbean 1 surprise…. Gobidae* Apogonidae* Coryphopterus eidolon C. hyalinusApogon townsendi Apogon maculatus   C. venezuelae C. thrix Apogon lachneri  Astrapogon puncticulatus C. tortugae Apogon mosavi Priolepis hipoliti Lythypnus sp *greatest number of species
  21. 21. Tripterygiidae Enneanectes altivelis Enneanectes boehlkei Labrisomidae  mmm…delicius! Malacoctenus triangulatus Starksia ocellata Labridae Thalassoma bifasciatum Halichoeres garnoti most frecuently we found…Pomacentridae Abudefduf sexatilis Stegastes partitus comelonas
  22. 22. Grammatidae Monacanthidae Gramma loreto Monacanthus tuckeri Pterois volitans Haemulidae Serranidae Haemulon flavolineatum Lioproma rubre Cephalopholis cruentata Chak-chi cabrilla Scaridae Loros Sparisoma viridae Scarus iserti Sparisoma aurofrenatum species economically important
  23. 23. Crustacean45 sequencesUsing the barcodes wecould identify 20 differentspecies.3 Orders:Euphausiacea…..1 speciesStomatopoda……2 speciesDecapoda………17 species
  24. 24. Euphausiacea Euphausia americana Euphausia spStomatopoda Pseudosquilla sp. This only was able to assign to family level… Gonodactylidae…
  25. 25. Decapoda: 17 species Hyppolytidae Thor sp. Alpheidae (CRU120) Palaemonidae Something like this….
  26. 26. In BoldDecapoda: 141 Familiesand 2,049 sp sequencedThe BOLD data base is notcomplete yet….
  27. 27. Conclusions1. This is an example of how important the barcodes are in the support of sustainable fisheries.2. With the help of barcodes we will know when and where the fish spawn, by directing protection measures in these particular seasons and sites we well suport fisheries.3. This is the first time we have a tool that will help us to identify all species targeted by lion fish.4. This is the first time that we have been able to report the kind of prey the lionfish consumes in the Mexican Caribbean.5. The only limit has been that the barcode library is still incomplete, mainly crustaceans, but with time it will be possible to identify all species consumed, not only by exotics, but also by any other species.
  28. 28. Acknowledgements:NOAA y RSMASCONANP – Xcalak y Banco ChinchorroCONABIO y BARCODE of LIFEECOSURITCH Jacc Diana Manuel Caro Lulú Selene Paty
  29. 29. Thanks for your attention!We need to visitthese places…. Xcalak
  1. A particular slide catching your eye?

    Clipping is a handy way to collect important slides you want to go back to later.

×