John La Salle - Opening Plenary
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John La Salle - Opening Plenary

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The “Barcode Blitz”: accelerating the targeted capture of barcode data

The “Barcode Blitz”: accelerating the targeted capture of barcode data

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    John La Salle - Opening Plenary John La Salle - Opening Plenary Presentation Transcript

    • The “Barcode Blitz”: accelerating the targeted capture of barcode data John La Salle, Beth Mantle CSIRO Ecosystem Sciences, Canberra, Australia Jeff Webb, Rodolphe Rougerie, Paul Hebert Biodiversity Institute of Ontario, Guelph Canada
    • The “Barcode Blitz” - ANIC Lepidoptera
        • A coordinated assault for targeted and priority capture of barcode data on ANIC Lepidoptera
        • Has shown it is possible to gather large amounts of data quickly
        • Built a substantive library for a megadiverse group at continental scale in a short time frame
        • Fits into / supports
          • Large scale digitisation efforts
          • Research outcomes
          • Operational outcomes
          • Rapid species discovery
    • The “Barcode Blitz”
        • Two visits from BIO / Guelph team to ANIC
        • 5 people x 5 weeks each
        • Jaclyn McCormick Paul Hebert
        • Jayme Sones Rodolphe Rougerie
        • Stephanie deWaard Jeff Webb
        • Cirsty Carr Jeremy deWaard
        • Renee Labbee Patrick Strutzenberger
        • Valerie Levesque-Beaudin Mari Kekkonen
    • The “Barcode Blitz”
        • In 10 weeks
        • 28,000 specimens processed
        • 8,000 species
        • Databased, geocoded, imaged on site
        • Leg taken
        • Sequenced in Canada
    • Assembly line processing
    • Assembly line processing
    • Selecting specimens
        • Multiple specimens per OTU
    • Selecting specimens
        • From collection to array box
    • Specimens into array boxes
        • Each space numbered
        • Aligns with number of wells in lysis plate
        • Position of every specimen taken from collection marked
    • Specimens databased and geocoded
    • Specimens into processing boxes
        • Specimens labelled as they are processed
          • BOLD label
          • ANIC label
    • Every specimen photographed
    • Leg removed for barcoding
        • Each lysis plate gets unique label that ties it back to array box
    • Specimens back to collection
        • Specimens returned to exactly where they were taken
    • The “Barcode Blitz”
        • In 10 weeks
        • 28,000 specimens processed
        • 8,000 species
        • Databased, geocoded, imaged on site
        • Uploaded to BOLD within 24 hours
        • Leg taken
        • Sequenced in Canada
    • The “Barcode Blitz” - what did we achieve Despite an average age of 30 years, COI sequences were recovered from more than 95% of the specimens
    • The “Barcode Blitz” - what did we achieve Provided the first continent-wide data set for a megadiverse insect group in Australia within a short period of time (about 90% of named Lepidoptera) Provides a model for inclusion in other initiatives aimed at the high-throughput accumulation of priority data sets. Establishes the great value of existing natural history collections as a basis for fast tracking the development of comprehensive DNA barcode libraries.
    • Despite popular opinion …
    • The “Barcode Blitz” - Australian benefits
        • Digital records in ANIC, ALA, BOLD
          • (28,000 databased, geocoded, imaged specimens)
    • The “Barcode Blitz” - results
        • What did we achieve
    • The “Barcode Blitz” - results
        • What did we achieve
    • The “Barcode Blitz” - Australian benefits
        • ANIC barcoding blitz activity is our clearest and most positive example of an accelerated and serious attempt to digitise a significant representative sample of a national collection.
        • Presenting the results in their fullness (including the capability of this process to highlight the range of undescribed diversity) would be a key element in a case to support a major national digitisation activity
    • One example of international benefit
        • A message from South Africa
        • “ Note that the mitochondrion sequences we obtained from the groundnut leaf miner in South Africa matched 100% with the soybean moth Approaerema simplexella PS1 from Australia on the BOLD system, and that is the reason we are interested in the soybean moth in Australia.”
    • The “Barcode Blitz” - Australian benefits
        • A searchable digital library for Australian Lepidoptera (including images and barcoding)
        • Foundation for a variety further studies
        • Facilitate identification
        • Able to connect life histories
        • Informs systematics, biodiversity, biosecurity
        • Foundation for Rapid Taxonomy efforts
    • Rapid Taxonomy of Australian Xylorictidae
        • Generations of investment in morphology (through putative species sorted in the collection)
        • Estimated 250 new species – with most of them already sorted to morphospecies
    • Rapid Taxonomy of Australian Xylorictidae
        • Group with no active specialist (last description in 1964) – no revisions on horizon.
        • Other major museums have made types of Australian xylorictids available for barcoding/study
        • Become a project for Patrick Strutzenberger (PhD student, Guelph)
        • DNA barcodes will be used to verify/validate morphospecies in collection
        • Produce standardized descriptions + images + barcodes (?plus genitalia)
        • Can progress taxonomic knowledge of this group rapidly
        • GOAL: 250 species described in 2 years, with 100 done by this time next year
    • Accelerated Species Discovery
        • taxonomist
        • Accelerated phenomics
        • Accelerated DNA
        • Accelerated species discovery and description
        • Crowdsourcing citizen scientists
    • Thank you Funding: Genome Canada CSIRO Transformational Biology
      • Bio Team from Guelph:
        • Jaclyn McCormick Rodolphe Rougerie
        • Jayme Sones Jeff Webb
        • Stephanie deWaard Jeremy deWaard
        • Cirsty Carr Patrick Strutzenberger
        • Renee Labbee Mari Kekkonen
        • Valerie Levesque-Beaudin
      Contact Us Phone: 1300 363 400 or +61 3 9545 2176 Email: enquiries@csiro.au Web: www.csiro.au