Dario Lijtmaer - Brief introduction to barcoding and the current goals and campaigns/working groups (iBOL context)
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Dario Lijtmaer - Brief introduction to barcoding and the current goals and campaigns/working groups (iBOL context)

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A brief introduction to barcoding and the current goals and campaigns/working groups (iBOL context)

A brief introduction to barcoding and the current goals and campaigns/working groups (iBOL context)

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Dario Lijtmaer - Brief introduction to barcoding and the current goals and campaigns/working groups (iBOL context) Dario Lijtmaer - Brief introduction to barcoding and the current goals and campaigns/working groups (iBOL context) Presentation Transcript

  • Short course on DNA barcoding methods November 29, 2011 OVERVIEW
  • Overview of this training session9:00 – 10:00. Module I: Introduction to Barcoding and its pipeline-Brief introduction to barcoding and the current goals, iBOL workinggroups and campaigns (Darío Lijtmaer)-The barcoding pipeline (Amy Driskell)-Barcode Data Standard compliance (David Schindel)
  • Overview of this training session10:00 – 12:30. Module II: Acquisition and Handling of specimensand tissue samples- Pros and cons of collecting specimens for barcoding vs. samplingexisting collections (Sally Adamowicz)- Field Collection (Sally Adamowicz)- Museum Harvesting (Jeremy deWaard)- Front-end processing (Michelle Van der Bank)
  • Overview of this training session14:00 – 15:30. Module III: Brief description of laboratory methodsand information management for small, medium and large-scalefacilities-DNA Extraction (Darío Lijtmaer)-PCR Amplification (Darío Lijtmaer)-Information management and data quality (Amy Driskell)
  • Overview of this training session16:00 – 17:30. Module IV: Taxon-specific aspects of the barcodingpipeline-Short talks about taxon-specific key elements -Plants (Dave Erickson) - Algae (Gary Saunders) - Fungi (Benjamin Stielow) - Marine invertebrates (Dirk Steinke) - Terrestrial invertebrates (Julie Stahlhut) - Vertebrates (Bob Ward)- Round table for Q&A and discussions about taxon-specific aspectsof the pipeline
  • Short course on DNA barcoding methods November 29, 2011Brief introduction to barcoding and the current goals, iBOL working groups and campaigns Darío Lijtmaer Museo Argentino de Ciencias Naturales “Bernardino Rivadavia”
  • Problems with traditional, morphology-based identification There are 10 -100 million species in our planet Species are variable Genetic-based identification (DNA barcodes) can help There are cryptic species overcoming these problemsSometimes we can only access fragments
  • The ideal genetic marker  Universal application  Good recoverability  Efficiency (smallest read necessary)  Easy to align  Easy to analyze The Mitochondrial Genome D-Loop Small ribosomal RNA Cytb ND6 COI ND2 L-strand H-strand COII COIII
  • Organization of the project: CBOLCBOL was established in 2004. Goals:- help organizing the project.- connect the members of the barcoding community.- establish barcoding as a standard proceduree for species identification. NMNH, Washington Distribution of CBOL institutions
  • Organization of the project: iBOLiBOL was officially launched in 2009.Main goal: to generate 5 million barcodes from 500,000 species by 2014. Central nodes Regional nodes National nodes
  • iBOL organization Theme 1: DNA Barcode Library WG1.1 Vertebrates WG1.2 Land Plants WG1.3 Fungi WG1.4 Human Pathogens and Zoonoses WG1.5 Agricultural and Forestry Pests WG1.6 Pollinators WG1.7 Freshwater Bio-Surveillance WG1.8 Marine Bio-Surveillance WG1.9 Terrestrial Bio-Surveillance WG1.10 Polar Life
  • iBOL organization Theme 2: Methodologies WG2.1 Barcoding Biotas WG2.2 Museum Life WG2.3 Methodological Innovation WG2.4 Paleobarcoding
  • WG2.3 Methodological Innovation
  • WG2.4 Paleobarcoding: Surveying the Past
  • iBOL organization Theme 3: Informatics WG3.1 Core Functionality WG3.2 Mirrors
  • iBOL organization Theme 4: Technologies WG4.1 Environmental Barcoding WG4.2 Handheld Barcoder
  • WG4.1 Environmental Barcoding:Species identifications from ‘slurries’
  • Agrawal et al 2007. A Pocket-Sized ConvectivePCR Thermocycler.
  • iBOL organization Theme 5: Administration WG5.1 Project management WG5.2 Communications
  • iBOL organization Theme 6: GE3LS Genomics and its Ethical, Environmental, Economic, Legal and Social aspects
  • iBOL organization Theme 1: DNA Barcode Library WG1.1 Vertebrates WG1.2 Land Plants WG1.3 Fungi WG1.4 Human Pathogens and Zoonoses WG1.5 Agricultural and Forestry Pests WG1.6 Pollinators WG1.7 Freshwater Bio-Surveillance WG1.8 Marine Bio-Surveillance WG1.9 Terrestrial Bio-Surveillance WG1.10 Polar Life
  • iBOL organization Theme 1: DNA Barcode Library WG1.1 Vertebrates WG1.2 Land Plants WG1.3 Fungi WG1.4 Human Pathogens and Zoonoses WG1.5 Agricultural and Forestry Pests WG1.6 Pollinators WG1.7 Freshwater Bio-Surveillance WG1.8 Marine Bio-Surveillance WG1.9 Terrestrial Bio-Surveillance WG1.10 Polar Life
  • iBOL organization Theme 1: DNA Barcode Library WG1.1 Vertebrates WG1.2 Land Plants WG1.3 Fungi WG1.4 Human Pathogens and Zoonoses WG1.5 Agricultural and Forestry Pests WG1.6 Pollinators WG1.7 Freshwater Bio-Surveillance WG1.8 Marine Bio-Surveillance WG1.9 Terrestrial Bio-Surveillance WG1.10 Polar Life
  • iBOL organization Theme 1: DNA Barcode Library WG1.1 Vertebrates WG1.2 Land Plants WG1.3 Fungi WG1.4 Human Pathogens and Zoonoses WG1.5 Agricultural and Forestry Pests WG1.6 Pollinators WG1.7 Freshwater Bio-Surveillance WG1.8 Marine Bio-Surveillance WG1.9 Terrestrial Bio-Surveillance WG1.10 Polar Life
  • iBOL organization Theme 1: DNA Barcode Library WG1.1 Vertebrates WG1.2 Land Plants WG1.3 Fungi WG1.4 Human Pathogens and Zoonoses WG1.5 Agricultural and Forestry Pests WG1.6 Pollinators WG1.7 Freshwater Bio-Surveillance WG1.8 Marine Bio-Surveillance WG1.9 Terrestrial Bio-Surveillance WG1.10 Polar Life
  • iBOL organization Theme 1: DNA Barcode Library WG1.1 Vertebrates WG1.2 Land Plants WG1.3 Fungi WG1.4 Human Pathogens and Zoonoses WG1.5 Agricultural and Forestry Pests WG1.6 Pollinators WG1.7 Freshwater Bio-Surveillance WG1.8 Marine Bio-Surveillance WG1.9 Terrestrial Bio-Surveillance WG1.10 Polar Life
  • iBOL organization Theme 1: DNA Barcode Library WG1.1 Vertebrates WG1.2 Land Plants WG1.3 Fungi WG1.4 Human Pathogens and Zoonoses WG1.5 Agricultural and Forestry Pests WG1.6 Pollinators WG1.7 Freshwater Bio-Surveillance WG1.8 Marine Bio-Surveillance WG1.9 Terrestrial Bio-Surveillance WG1.10 Polar Life
  • Why are we here today? Specimen Collection data Sample Photographs Extract Amplify Sequence
  • Thank you!Questions?