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DNA barcoding with reference to marine vertebrates.

DNA barcoding with reference to marine vertebrates.

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  • 1. Fish ‘barcode blitz’ at Lizard Island (Great Barrier Reef)
  • 2. Vertebrate COI barcode primers Commonly used primers Fish CO1-3, CO1-2, (FishF1, FishF2, FishR1, FishR2) Amphibia COI-3, dgLCO/dgHCO,(M)LepF1/LepR1 Reptiles COI-3 Birds BirdF1/CObirdR2, BirdF1/BirdR1 Mammals COI-2, COI-3 (FishF1, FishR1) FishF1, FishF2, FishR1 and FishR2 primer details in Ward et al. 2005. COI-2 and CO1-3 cocktail primer details in Ivanova et al. 2007. Sequences of other primers are available through BOLD.FISH TISSUES USED:White muscle tissueFin clipsEyes (from tiny or larval fish)
  • 3. Vertebrate COI barcoding progress Number of Barcodes* % species species (2010) Specimens Species barcodedFish 31,300 103,904 12,114 38.7Amphibia 6,433 9,148 1,291 20.1Reptiles 9,084 4,636 1,125 12.4Birds 9,998 27,740 4,194 41.9Mammals 5,490 42,769 1,976 36.0TOTAL 62,305 188,197 20,700 33.2*from BOLD Taxonomy Browser (14 Nov 2011)
  • 4. Vertebrate COI barcoding progress Number of Barcodes* % species species (2010) Specimens Species barcodedFish 31,300 103,904 12,114 38.7Amphibia 6,433 9,148 1,291 20.1Reptiles 9,084 4,636 1,125 12.4Birds 9,998 27,740 4,194 41.9Mammals 5,490 42,769 1,976 36.0TOTAL 62,305 188,197 20,700 33.2*from BOLD Taxonomy Browser (14 Nov 2011)Fish have a unique (?) vertebrate problem of often beingdifficult to identify, and with many cryptic undescribed species.