Your SlideShare is downloading. ×
  • Like
Biocroi web presentation 07 03-2012
Upcoming SlideShare
Loading in...5
×

Thanks for flagging this SlideShare!

Oops! An error has occurred.

×

Now you can save presentations on your phone or tablet

Available for both IPhone and Android

Text the download link to your phone

Standard text messaging rates apply

Biocroi web presentation 07 03-2012

  • 382 views
Published

The latest concept in microplate design involving a gel base technology. The full functionality of conventional microplates with environmental buffering technology on-board reducing edge-effects and …

The latest concept in microplate design involving a gel base technology. The full functionality of conventional microplates with environmental buffering technology on-board reducing edge-effects and permitting extreme miniaturisation all in existing SBS format

Published in Technology , Business
  • Full Name Full Name Comment goes here.
    Are you sure you want to
    Your message goes here
    Be the first to comment
    Be the first to like this
No Downloads

Views

Total Views
382
On SlideShare
0
From Embeds
0
Number of Embeds
1

Actions

Shares
Downloads
3
Comments
0
Likes
0

Embeds 0

No embeds

Report content

Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

Cancel
    No notes for slide
  • All chemical and biological processes are influenced by environmental elements such as temperature, pH and chemical composition. Uncontrolled changes in any of these factors can exert unwanted physical, chemical and/or biological effects on the specimen/sample in question leading to poor reproducibility or disruption of a given scientific and/or manufacturing process With the emergence of high throughput and multiplexed biological, chemical and materials screening, the use of multi-welled assay plates has become standard for almost all automated experimental and storage applications. However many of these experimental procedures compromised by positional inequalities between wells on outer and inner locations on the micro-plate. These inequalities or “edge effects” become increasingly apparent as micro-plate densities increase. To improve reproducibility when conducting chemical and biological experiments, optimal environmental conditions must be maintained at all times. To achieve this, it is often necessary to deploy costly and cumbersome environmental control systems which dramatically reduce experimental design flexibility automated screening platforms, often these interventions do not solve the problem.
  • All chemical and biological processes are influenced by environmental elements such as temperature, pH and chemical composition. Uncontrolled changes in any of these factors can exert unwanted physical, chemical and/or biological effects on the specimen/sample in question leading to poor reproducibility or disruption of a given scientific and/or manufacturing process With the emergence of high throughput and multiplexed biological, chemical and materials screening, the use of multi-welled assay plates has become standard for almost all automated experimental and storage applications. However many of these experimental procedures compromised by positional inequalities between wells on outer and inner locations on the micro-plate. These inequalities or “edge effects” become increasingly apparent as micro-plate densities increase. To improve reproducibility when conducting chemical and biological experiments, optimal environmental conditions must be maintained at all times. To achieve this, it is often necessary to deploy costly and cumbersome environmental control systems which dramatically reduce experimental design flexibility automated screening platforms, often these interventions do not solve the problem.
  • All chemical and biological processes are influenced by environmental elements such as temperature, pH and chemical composition. Uncontrolled changes in any of these factors can exert unwanted physical, chemical and/or biological effects on the specimen/sample in question leading to poor reproducibility or disruption of a given scientific and/or manufacturing process With the emergence of high throughput and multiplexed biological, chemical and materials screening, the use of multi-welled assay plates has become standard for almost all automated experimental and storage applications. However many of these experimental procedures compromised by positional inequalities between wells on outer and inner locations on the micro-plate. These inequalities or “edge effects” become increasingly apparent as micro-plate densities increase. To improve reproducibility when conducting chemical and biological experiments, optimal environmental conditions must be maintained at all times. To achieve this, it is often necessary to deploy costly and cumbersome environmental control systems which dramatically reduce experimental design flexibility automated screening platforms, often these interventions do not solve the problem.

Transcript

  • 1. At the cutting edge of cell based assaysGel Base BioreactorTechnologyMarch 2012CONFIDENTIALITY NOTICE Biocroí Ltd.This document is the property of Biocroí Ltd and contains confidential information. This document shall not be reproduced, Unit 3, 80 The Trinity Centrecopied, or used for any purposes without the express written permission of an authorized representative of the company. St James’s Hospital, Dublin 8, Ireland
  • 2. The problemCONFIDENTIALITY NOTICE Biocroí Ltd.This document is the property of Biocroí Ltd and contains confidential information. This document shall not be reproduced, Unit 3, 80 The Trinity Centrecopied, or used for any purposes without the express written permission of an authorized representative of the company. St James’s Hospital, Dublin 8, Ireland
  • 3. All chemical and biological processes are influenced byenvironmental elements such as temperature, pH andchemical composition.CONFIDENTIALITY NOTICE Biocroí Ltd.This document is the property of Biocroí Ltd and contains confidential information. This document shall not be reproduced, Unit 3, 80 The Trinity Centrecopied, or used for any purposes without the express written permission of an authorized representative of the company. St James’s Hospital, Dublin 8, Ireland
  • 4. Environmental Concerns Fluctuations Variations in in thermal dissolved CO2 conditions Cell Based Assays Mechanical Alterations in disturbances water media (Vibrations etc) hydrationCONFIDENTIALITY NOTICE Biocroí Ltd.This document is the property of Biocroí Ltd and contains confidential information. This document shall not be reproduced, Unit 3, 80 The Trinity Centrecopied, or used for any purposes without the express written permission of an authorized representative of the company. St James’s Hospital, Dublin 8, Ireland
  • 5. Uncontrolled changes in any of these factors can exert unwantedphysical, chemical and/or biological effects on thespecimen/sample in question leading to poor reproducibility ordisruption of a given scientific and/or manufacturing processCONFIDENTIALITY NOTICE Biocroí Ltd.This document is the property of Biocroí Ltd and contains confidential information. This document shall not be reproduced, Unit 3, 80 The Trinity Centrecopied, or used for any purposes without the express written permission of an authorized representative of the company. St James’s Hospital, Dublin 8, Ireland
  • 6. Negative outcomes Increased Cellular Heterogeneity stress in cell populations Disruption & Poor Reproducibility Aberrant Retardation gene of growth expressionCONFIDENTIALITY NOTICE Biocroí Ltd.This document is the property of Biocroí Ltd and contains confidential information. This document shall not be reproduced, Unit 3, 80 The Trinity Centrecopied, or used for any purposes without the express written permission of an authorized representative of the company. St James’s Hospital, Dublin 8, Ireland
  • 7. With the emergence of high throughput and multiplexedbiological, chemical and materials screening, the use of multi-welled assay plates has become standard for almost all automatedexperimental and storage applications.CONFIDENTIALITY NOTICE Biocroí Ltd.This document is the property of Biocroí Ltd and contains confidential information. This document shall not be reproduced, Unit 3, 80 The Trinity Centrecopied, or used for any purposes without the express written permission of an authorized representative of the company. St James’s Hospital, Dublin 8, Ireland
  • 8. However many of these experimental procedures arecompromised by positional inequalities between wells on outerand inner locations on the micro-plate.These inequalities or “edge effects” become increasingly apparentas micro-plate densities increase.CONFIDENTIALITY NOTICE Biocroí Ltd.This document is the property of Biocroí Ltd and contains confidential information. This document shall not be reproduced, Unit 3, 80 The Trinity Centrecopied, or used for any purposes without the express written permission of an authorized representative of the company. St James’s Hospital, Dublin 8, Ireland
  • 9. To improve reproducibility when conducting chemical andbiological experiments, optimal environmental conditions must bemaintained at all times.To achieve this, it is often necessary to deploy costly andcumbersome environmental control systems. These systemsdramatically reduce the experimental design flexibility ofautomated screening platforms and often do not solve theproblem.CONFIDENTIALITY NOTICE Biocroí Ltd.This document is the property of Biocroí Ltd and contains confidential information. This document shall not be reproduced, Unit 3, 80 The Trinity Centrecopied, or used for any purposes without the express written permission of an authorized representative of the company. St James’s Hospital, Dublin 8, Ireland
  • 10. Our solutionCONFIDENTIALITY NOTICE Biocroí Ltd.This document is the property of Biocroí Ltd and contains confidential information. This document shall not be reproduced, Unit 3, 80 The Trinity Centrecopied, or used for any purposes without the express written permission of an authorized representative of the company. St James’s Hospital, Dublin 8, Ireland
  • 11. Thermal Improved Buffering Cell Growth Phase-lock Technology Prevents Better evaporation well to well consistencyCONFIDENTIALITY NOTICE Biocroí Ltd.This document is the property of Biocroí Ltd and contains confidential information. This document shall not be reproduced, Unit 3, 80 The Trinity Centrecopied, or used for any purposes without the express written permission of an authorized representative of the company. St James’s Hospital, Dublin 8, Ireland
  • 12. Side view and plan of gel plate showing construction of wells of plate surrounded by gel. Plate Gel WellsCONFIDENTIALITY NOTICE Biocroí Ltd.This document is the property of Biocroí Ltd and contains confidential information. This document shall not be reproduced, Unit 3, 80 The Trinity Centrecopied, or used for any purposes without the express written permission of an authorized representative of the company. St James’s Hospital, Dublin 8, Ireland
  • 13. Loading in Incubator Un-loading out of incubator Heat, CO2 passes into Gel down thermal and concentration gradientCONFIDENTIALITY NOTICE Biocroí Ltd.This document is the property of Biocroí Ltd and contains confidential information. This document shall not be reproduced, Unit 3, 80 The Trinity Centrecopied, or used for any purposes without the express written permission of an authorized representative of the company. St James’s Hospital, Dublin 8, Ireland
  • 14. A 384 well gel buffered bioreactorCONFIDENTIALITY NOTICE Biocroí Ltd.This document is the property of Biocroí Ltd and contains confidential information. This document shall not be reproduced, Unit 3, 80 The Trinity Centrecopied, or used for any purposes without the express written permission of an authorized representative of the company. St James’s Hospital, Dublin 8, Ireland
  • 15. Top view of the 384bioreactorplatewith gel bufferingCONFIDENTIALITY NOTICE Biocroí Ltd.This document is the property of Biocroí Ltd and contains confidential information. This document shall not be reproduced, Unit 3, 80 The Trinity Centrecopied, or used for any purposes without the express written permission of an authorized representative of the company. St James’s Hospital, Dublin 8, Ireland
  • 16. Thermal bufferingCONFIDENTIALITY NOTICE Biocroí Ltd.This document is the property of Biocroí Ltd and contains confidential information. This document shall not be reproduced, Unit 3, 80 The Trinity Centrecopied, or used for any purposes without the express written permission of an authorized representative of the company. St James’s Hospital, Dublin 8, Ireland
  • 17. 1.1 1 0.9 0.8 0.7 0.6 Cooling rate reduced tx/t0 % 0.5 0.4 Thermal retention ~ 3 X Improved 0.3 SBS format plate 0.2 0.1 0 0 5 10 15 20 25 30 35 Time (mins) Comparison of cooling between normal and Biocroí platesCONFIDENTIALITY NOTICE Biocroí Ltd.This document is the property of Biocroí Ltd and contains confidential information. This document shall not be reproduced, Unit 3, 80 The Trinity Centrecopied, or used for any purposes without the express written permission of an authorized representative of the company. St James’s Hospital, Dublin 8, Ireland
  • 18. Protection against evaporationCONFIDENTIALITY NOTICE Biocroí Ltd.This document is the property of Biocroí Ltd and contains confidential information. This document shall not be reproduced, Unit 3, 80 The Trinity Centrecopied, or used for any purposes without the express written permission of an authorized representative of the company. St James’s Hospital, Dublin 8, Ireland
  • 19. Wells of 96 welledplates were filled with100ul serum freeculture medium andthen maintained in astandard drying ovenset at 50ºC for 48hours. (Dataexpressed aspercentage volumeremaining afterincubation time.(See plate diagram toidentify wells used inall experiments).CONFIDENTIALITY NOTICE Biocroí Ltd.This document is the property of Biocroí Ltd and contains confidential information. This document shall not be reproduced, Unit 3, 80 The Trinity Centrecopied, or used for any purposes without the express written permission of an authorized representative of the company. St James’s Hospital, Dublin 8, Ireland
  • 20. Improved cell growth& reproducibilityCONFIDENTIALITY NOTICE Biocroí Ltd.This document is the property of Biocroí Ltd and contains confidential information. This document shall not be reproduced, Unit 3, 80 The Trinity Centrecopied, or used for any purposes without the express written permission of an authorized representative of the company. St James’s Hospital, Dublin 8, Ireland
  • 21. Buffered Plates Normal PlatesWells of buffered and normal 96 well plates were seeded at (equally) low density with cells of immortalised cell line.Prior to maintenance in a standard tissue culture incubator (set at 37C, 95% air/ 5% CO2) for 48 hours. Both bufferedand normal plates were placed in middle of incubator side by side.CONFIDENTIALITY NOTICE Biocroí Ltd.This document is the property of Biocroí Ltd and contains confidential information. This document shall not be reproduced, Unit 3, 80 The Trinity Centrecopied, or used for any purposes without the express written permission of an authorized representative of the company. St James’s Hospital, Dublin 8, Ireland
  • 22. Non-insulated 96 well Insulated 96 well14710 (1871) 16805 (1029) 12.7% 6.12% DRASTICCV= REDUCTION OF EDGE EFFECTS CV=CONFIDENTIALITY NOTICE Biocroí Ltd.This document is the property of Biocroí Ltd and contains confidential information. This document shall not be reproduced, Unit 3, 80 The Trinity Centrecopied, or used for any purposes without the express written permission of an authorized representative of the company. St James’s Hospital, Dublin 8, Ireland
  • 23. The excellent environmental buffering properties of Biocroí platetechnologies allows miniaturisation of cell based assays down tonanoliter volumes.CONFIDENTIALITY NOTICE Biocroí Ltd.This document is the property of Biocroí Ltd and contains confidential information. This document shall not be reproduced, Unit 3, 80 The Trinity Centrecopied, or used for any purposes without the express written permission of an authorized representative of the company. St James’s Hospital, Dublin 8, Ireland
  • 24. High Content Image of cells reverse transfected with SiGlo in a gel buffered 192 nano slide. (Volume 100nl) Miniaturised 1000X As compared to 96- well plate assays normally performed at 100ulCONFIDENTIALITY NOTICE Biocroí Ltd.This document is the property of Biocroí Ltd and contains confidential information. This document shall not be reproduced, Unit 3, 80 The Trinity Centrecopied, or used for any purposes without the express written permission of an authorized representative of the company. St James’s Hospital, Dublin 8, Ireland
  • 25. High Content Image of cells incubated in 100nl in a standard 384-well buffered plateCONFIDENTIALITY NOTICE Biocroí Ltd.This document is the property of Biocroí Ltd and contains confidential information. This document shall not be reproduced, Unit 3, 80 The Trinity Centrecopied, or used for any purposes without the express written permission of an authorized representative of the company. St James’s Hospital, Dublin 8, Ireland
  • 26. Reproducibility of spotting 50 nanoliter siRNA in 384 buffered gel plateCONFIDENTIALITY NOTICE Biocroí Ltd.This document is the property of Biocroí Ltd and contains confidential information. This document shall not be reproduced, Unit 3, 80 The Trinity Centrecopied, or used for any purposes without the express written permission of an authorized representative of the company. St James’s Hospital, Dublin 8, Ireland
  • 27. Dried 50 nanoliter siRNA spot overlain with 100 nanoliter lung carcinoma cellsCONFIDENTIALITY NOTICE Biocroí Ltd.This document is the property of Biocroí Ltd and contains confidential information. This document shall not be reproduced, Unit 3, 80 The Trinity Centrecopied, or used for any purposes without the express written permission of an authorized representative of the company. St James’s Hospital, Dublin 8, Ireland
  • 28. AdvantagesCONFIDENTIALITY NOTICE Biocroí Ltd.This document is the property of Biocroí Ltd and contains confidential information. This document shall not be reproduced, Unit 3, 80 The Trinity Centrecopied, or used for any purposes without the express written permission of an authorized representative of the company. St James’s Hospital, Dublin 8, Ireland
  • 29. Increase in Proven reduction throughput in edge effects productivity Substantial Improved data decrease in quality reagent & cell usageCONFIDENTIALITY NOTICE Biocroí Ltd.This document is the property of Biocroí Ltd and contains confidential information. This document shall not be reproduced, Unit 3, 80 The Trinity Centrecopied, or used for any purposes without the express written permission of an authorized representative of the company. St James’s Hospital, Dublin 8, Ireland