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iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
iGEM 2013 NTU-Taida Presentation Part 2 修改後範例
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iGEM 2013 NTU-Taida Presentation Part 2 修改後範例

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  • 1. Overview Circuit Result Modeling Human Practice
  • 2. Three Circuit Types GFP 1.Original GFP GFP GFP 2.Positive Feedback Faster & Higher Intensity GFP GFP GFP 3.Negative Regulation More Stable Overview Circuit Result Modeling Human Practice
  • 3. 1.Original Circuit Induced Promoter RBS Reporter Double Terminator Constitutive Promoter QS Receptor GFP Overview Circuit Result Modeling Human Practice Double Terminator
  • 4. 1.Original Circuit Induced Promoter RBS Reporter Double Terminator Constitutive Promoter QS Receptor GFP Overview Circuit Result Modeling Human Practice Double Terminator
  • 5. 1.Original Circuit Induced Promoter RBS Reporter Double Terminator Constitutive Promoter QS Receptor GFP Overview Circuit Result Modeling Human Practice Double Terminator
  • 6. 1.Original Circuit Induced Promoter RBS Reporter Double Terminator Constitutive Promoter QS Receptor GFP Overview Circuit Result Modeling Human Practice Double Terminator
  • 7. 1.Original Circuit Induced Promoter RBS Reporter Double Terminator Constitutive Promoter QS Receptor GFP Overview Circuit Result Modeling Human Practice Double Terminator
  • 8. 1.Original Circuit Induced Promoter RBS Reporter Double Terminator Constitutive Promoter QS Receptor GFP Overview Circuit Result Modeling Human Practice Double Terminator
  • 9. 1.Original Circuit Induced Promoter RBS Reporter Double Terminator Constitutive Promoter QS Receptor GFP Overview Circuit Result Modeling Human Practice Double Terminator
  • 10. 1.Original Circuit Induced Promoter RBS Reporter Double Terminator Constitutive Promoter QS Receptor GFP GFP Overview Circuit Result Modeling Human Practice Double Terminator
  • 11. ELISA Plate Reader Overview Circuit Result Modeling Human Practice
  • 12. ELISA Plate Reader Overview Circuit Result Modeling Human Practice
  • 13. Flow Cytometry Start Overview Circuit Result Modeling Human Practice
  • 14. Flow Cytometry Start 1st Hour Overview Circuit Result Modeling Human Practice
  • 15. Flow Cytometry Start 1st Hour Overview 2nd Hour Circuit Result Modeling Human Practice
  • 16. Flow Cytometry Start 1st Hour Overview 2nd Hour Circuit Result 3rd Hour Modeling Human Practice
  • 17. Flow Cytometry Start 1st Hour Overview 2nd Hour Circuit Result 3rd Hour Modeling 4th Hour Human Practice
  • 18. 2. Positive Feedback Circuit GFP RhIR pRhI Overview Circuit Result Modeling Human Practice
  • 19. 2. Positive Feedback Circuit GFP RhIR pRhI Overview Circuit Result Modeling Human Practice
  • 20. 2. Positive Feedback Circuit RhIR GFP Overview Circuit Result RhIR pRhI Modeling Human Practice
  • 21. 2. Positive Feedback Circuit RhIR GFP RhIR pRhI RhIR Overview Circuit Result Modeling Human Practice
  • 22. 2. Positive Feedback Circuit RhIR GFP RhIR pRhI RhIR Overview Circuit Result Modeling Human Practice
  • 23. 2. Positive Feedback Circuit RhIR GFP RhIR pRhI RhIR RhIR Overview Circuit Result Modeling Human Practice
  • 24. 2. Positive Feedback Circuit RhIR GFP RhIR pRhI RhIR RhIR Overview Circuit Result Modeling Human Practice
  • 25. 2. Positive Feedback Circuit RhIR GFP RhIR pRhI GFP RhIR RhIR Overview Circuit Result Modeling Human Practice
  • 26. 2. Positive Feedback Circuit RhIR GFP RhIR pRhI GFP RhIR RhIR RhIR Overview Circuit Result Modeling Human Practice
  • 27. 2. Positive Feedback Circuit RhIR GFP RhIR pRhI GFP RhIR RhIR RhIR Positive Feedback Circuit Overview Circuit Result Modeling Human Practice
  • 28. ELISA Plate Reader Overview Circuit Result Modeling Human Practice
  • 29. ELISA Plate Reader Overview Circuit Result Modeling Human Practice
  • 30. Flow Cytometry Start Overview Circuit Result Modeling Human Practice
  • 31. Flow Cytometry Start 1st Hour Overview Circuit Result Modeling Human Practice
  • 32. Flow Cytometry Start 1st Hour Overview 2nd Hour Circuit Result Modeling Human Practice
  • 33. Flow Cytometry Start 1st Hour Overview 2nd Hour Circuit Result 3rd Hour Modeling Human Practice
  • 34. Flow Cytometry Start 1st Hour Overview 2nd Hour Circuit Result 3rd Hour Modeling 4th Hour Human Practice
  • 35. 3. Negative Regulation Divided into 3 separated steps: Overview Circuit Result Modeling Human Practice
  • 36. 3. Negative Regulation Divided into 3 separated steps: Positive Feedback Overview Circuit Result Modeling Human Practice
  • 37. 3. Negative Regulation Divided into 3 separated steps: Positive Feedback Overview Repressor Circuit Result Modeling Human Practice
  • 38. 3. Negative Regulation Divided into 3 separated steps: Positive Feedback Overview Repressor Circuit Result Modeling Reporter Human Practice
  • 39. 3. Negative Regulation Positive Feedback Repressor Reporter Overview Circuit Result Modeling Human Practice
  • 40. 3. Negative Regulation Double Terminator LasR pConst Positive Feedback Repressor Reporter Overview Circuit Result Modeling Human Practice
  • 41. 3. Negative Regulation Double Terminator LasR pConst Positive Feedback LasR Repressor Reporter Overview Circuit Result Modeling Human Practice
  • 42. 3. Negative Regulation Double Terminator LasR pConst LasR Positive Feedback AHL Repressor Reporter Overview Circuit Result Modeling Human Practice
  • 43. 3. Negative Regulation Double Terminator LasR pConst LasR Positive Feedback AHL LasR CI Repressor pLas Reporter Overview Circuit Result Modeling Human Practice
  • 44. 3. Negative Regulation Double Terminator LasR pConst LasR Positive Feedback AHL LasR CI Repressor pLas Reporter Overview Circuit Result Modeling Human Practice
  • 45. 3. Negative Regulation Double Terminator LasR pConst LasR Positive Feedback AHL LasR CI Repressor pLas CI Reporter Overview Circuit Result Modeling Human Practice
  • 46. 3. Negative Regulation Double Terminator Positive Feedback LasR pConst LasR AHL LasR Repressor CI pLas CI GFP pCI Reporter Luciferase/XFP Overview Circuit Result Modeling Human Practice
  • 47. 3. Negative Regulation Double Terminator Positive Feedback LasR pConst LasR AHL LasR Repressor CI pLas CI GFP pCI Reporter Luciferase/XFP Overview Circuit Result Modeling Human Practice
  • 48. 3. Negative Regulation Double Terminator Positive Feedback LasR pConst LasR AHL LasR Repressor CI pLas CI GFP pCI Reporter Luciferase/XFP Overview Circuit Result Modeling Human Practice
  • 49. 3. Negative Regulation Double Terminator Positive Feedback LasR pConst LasR AHL LasR Repressor CI pLas CI GFP pCI Reporter Luciferase/XFP Overview Circuit Result Modeling Human Practice
  • 50. ELISA Plate Reader Overview Circuit Result Modeling Human Practice
  • 51. ELISA Plate Reader Overview Circuit Result Modeling Human Practice
  • 52. Flow Cytometry Start Overview Circuit Result Modeling Human Practice
  • 53. Flow Cytometry Start 1st Hour Overview Circuit Result Modeling Human Practice
  • 54. Flow Cytometry Start 1st Hour Overview 2nd Hour Circuit Result Modeling Human Practice
  • 55. Flow Cytometry Start 1st Hour Overview 2nd Hour Circuit Result 3rd Hour Modeling Human Practice
  • 56. Flow Cytometry Start 1st Hour Overview 2nd Hour Circuit Result 3rd Hour Modeling 4th Hour Human Practice
  • 57. Comparison Of Three Circuits GFP 1.Original GFP GFP GFP 2.Positive Feedback Faster & Higher Intensity GFP GFP GFP 3.Negative Regulation More Stable Overview Circuit Result Modeling Human Practice
  • 58. Comparison Of Three Circuits Normal State GFP 1.Original GFP GFP GFP 2.Positive Feedback Faster & Higher Intensity GFP GFP GFP 3.Negative Regulation More Stable Overview Circuit Result Modeling Human Practice
  • 59. Comparison Of Three Circuits QS Signal GFP 1.Original GFP GFP GFP 2.Positive Feedback Faster & Higher Intensity GFP GFP GFP 3.Negative Regulation More Stable Overview Circuit Result Modeling Human Practice

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