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Cytoskelet Plus Matrix Deel 1
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Cytoskelet Plus Matrix Deel 1 Presentation Transcript

  • 1.  
  • 2.  
  • 3. The cytoskeleton Actin cytoskeleton: polymerisation of G-actin Microtubuli: Polymerisation of tubulin Intermediate filaments: Coiled-coiled structure of e.g. keratins Keratin filaments
  • 4. Green: F-actin Red: VE-cadherin (junction protein) F-actin can be stained specifically with phalloidin G-actin: monomers F-actin: filamentous
  • 5. Actin polymerisation at leading edge of cell: All F-actin Only newly polymerized F-actin Regulation in time and space!!
  • 6. Profilin recruits actin monomers: nucleation of actin
  • 7. Actin related protein (Arp) 2/3 complex: nucleation of actin
  • 8. Actin: Red Arp2/3: Green Arp 2/3 complex: branching of actin
  • 9. Actin treadmilling: cycle of polymerisation/depolymerisation
  • 10. Crosslinking proteins form networks
  • 11. Rho family members (Rac, Rho, CDC42) regulate localized actin polymerisation Effector GAP Rac GDP GEF Rac GTP Stimulus (e.g. chemotatic factor)
  • 12. Rho family members (Rac, Rho, CDC42) regulate localized actin polymerisation: Specificity !
  • 13. Figure 16-98a Molecular Biology of the Cell (© Garland Science 2008)
  • 14. Figure 16-98b Molecular Biology of the Cell (© Garland Science 2008)
  • 15. Polymerised actin is anchored to the plasma membrane: A regulated process
  • 16. Microtubuli are also highly dynamic GFP-EB1 (EB1 is a cap protein)
  • 17. Taxol stabilizes microtubuli: cytostatic agent
  • 18. Cell junctions
  • 19. Tight junctions : Functions: 1. Seal apical lumen from basal lumen: Regulated permeability 2. Barrier between apical and basolateral membrane Cell polarity Glucose transport in the gut: one direction only
  • 20. Claudins and Occludins are major component of TJ
  • 21. Adherence junctions Function Cell cell adhesion Connected to actin bundels: adhesion belt
  • 22. Adhesion belts involved in - keeping the integrity of the epithelial sheet - morphogenesis
  • 23. Cadherins are major component of AJ: Homotypic binding E-cadherin N-cadherin P-cadherin VE-cadherin
  • 24. Cadherins are connected to the actin cytoskeleton through catenines  -catenin Note:  -catenin functions independently in Wnt signalling pathway
  • 25. Cadherins involved in cell sorting Different cadherins Different expression levels
  • 26. Cell junctions are dynamic GFP-ZO-1 Ovcar3 cells Movie: Johan de Rooij
  • 27. Adhesion is regulated by recycling of cadherins Cell division Growth factors Oncogenes Vesicle transport Adherence junctions