EFFECT OF DRILLING MUD/FLUIDS ON MARINE MICRO-ORGANISMS

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This is one of the research proposal we presented at NEERI. This was about understanding the effect of drilling fluids on marine organisms

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EFFECT OF DRILLING MUD/FLUIDS ON MARINE MICRO-ORGANISMS

  1. 1. EFFECT OF DRILLINGMUD/FLUIDS ON MARINE MICRO-ORGANISMS Avick Sil, Kanchan Wakadikar and S. P. M Sivagami avick1411@gmail.com NEERI Mumbai Zonal Lab
  2. 2. OBJECTIVESIsolating and Culturing Marinemicro-organismsTo study effect of drilling fluid onMarine microorganismDegradation of Barite (BaSO4) usingnatural Marine micro-organismsTo study Growth profile of Marineorganism in presence of drillingfluids.Study of Heterotrophs
  3. 3. COMPONENTS OF DRILLING FLUID Major NAF Barite Emulsifier Brine Others Minor CaCl2 Lime (CaC03) Hot Lime (CaO) Organophilic Clay
  4. 4. EFFECT OF DRILLING FLUIDSSources Accidental spillage, offshore activities & standing of tankers Marine water Deteriorating water quality Oxygen depletion Fishes Microorganisms Mammals BirdsDeath Affects Affects insulating water- Reproduction & ability repelling F1 generation ability
  5. 5. MATERIALS &METHODS
  6. 6. ISOLATION AND ENUMERATION OF MARINE MICRO-ORGANISMS A. Study of Heterotrophs Isolate the microbes on Nutrient agar plates Enumerate the number of colony forming Units Replicate the colonies from nutrient agar plate to marine agar plateEnumerate and differences in microbial population will beassayed by plate count and by colony characterization
  7. 7. B. Study the effect of drilling mud on marine micro-organisms Different concentrations of drilling fluid will be prepared in Nutrient agar and Marine agar. Enumerate the number of survivals by colony countResults of Nutrient Agar Results of Marineindicates the effect on all Agar indicates the marine microbes effect on heterotrophs
  8. 8. EXPECTED RESULTS With increase in concentration of drilling fluids, the number of organisms will decrease.M. M. Al-Dabi, N. O. Saeed, M. R. Islam; Biodegradation of Natural Oils in Seawater; Faculty ofEngineering, Dalhousie University, Halifax, NS, Canada, K. LEE, Bedford Institute of OceanographyDartmouth, NS, Canada
  9. 9. C. Study on growth pattern of microorganisms Isolation of microbes on nutrient broth (Control) and on marine broth Growth profile will be assayed with different concentration of drilling fluid Growth profile will be determined by measuring the Optical density Generation time and growth curve of microorganisms will be calculated
  10. 10. Experimental setup for studying growth curve of Marine organisms
  11. 11. EXPECTED RESULTS ;C. O. Nweke1 and G. C. Okpokwasili Drilling fluid base oil biodegradation potential of a soilStaphylococcus species
  12. 12. D. Biodegradation of Barite Isolation and sub culturing of marine MicroorganismsPure culture will be maintained and it will be spot inoculated on media containing different concentration of barite.Same spot inoculation will be assayed for degradation of different drilling fluids
  13. 13. Media containing Spot inoculation Zone of inhibition drilling fluid/Barite Biodegradation of Drilling mud and Barite by isolated microorganisms
  14. 14. FLOW OF THE EXPERIMENT Isolation of marine microorganisms on Nutrient agar Replica plating on Marine agar Study of HeterotrophsCharacterization of each microorganisms by Gram nature andcolony characteristics and comparing it with Bergy’s manaul Maintenance of pure cultureStudy of Growth profile Study of Growth profile on Nutrient broth on Marine broth
  15. 15. Cont…..FLOW OF THE EXPERIMENTPreparation of different concentrations of Barite and Drilling Mud/Oil in Nutrient agar & Marine agar Spot inoculation of each organism Measurement of zone of inhibitions
  16. 16. ROLES AND RESPONSIBILITIESAvick1. Isolation on both Nutrient agar and Marine agar2. Replica plating 3. Heterotroph analysisKanchan1. Maintenance of pure culture 2. Analysis of growth curve3. Identification of each Microorganism using Study of Gram nature &colony characteristics & comparing it with Bergy’s manualSivagami1. Preparation of different concentration of Barite and Drilling fluid2. Analysis of Breakdown of Barite and Drilling Fluid with isolatesMicroorganims
  17. 17. Suggestions

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