Snake Venom Immunology Ivan J. Garcia Motta 804-10-2844 RISE BIO 3009 07-26-10
Introduction Snake Venom has been said to be a powerful immunology aditive. It has been tested on animals, but there is no clear evidence. Most anti-venoms are created from blood of a venom injected animal that created the antibodies to fight off the venom. Snakes have been involved in medicinal uses on certain cultures.
Problem A large number of the worlds population suffers from immunity defects and lack of antibodies. As well as a large number of people get bitten by snakes year-round. Diseases are causing a rapid decrease on immune systems.
Solution Anti-venom is venom diluted with other substances and stronger antibodies. This means that snake venom does posses medicinal uses and potential immunological use. Create a solution that, over time, will create stronger antibodies and a stronger immune system in human beings.
Hypothesis Injecting a diluted solution of snake venom (1:10, snake venom to water) over time will create stronger antibodies and a stronger immune system. After a year or so, the antibodies will grow and become stronger, as well as increase the bodies defenses. The toxins in snake venom are strong antibodies and strong proteins. These proteins will fight off diseases.
Methodology: Theory Snake venom is a type of modified saliva with toxins and added proteins and enzymes. The proteins and the toxins break down and kill the prey. Albert Calmette was the creator anti-venom. He did this by hyper-immunizing (large number of anti-bodies)an animal with a snakes venom, then extracting its blood, which served as a serum for anti-venom.
Previous Research Previously Neurotoxins have been used to treat arthritis and Alzheimer. Neurotoxins have been used widely in the field of medicine and science, and have shown improvements in its patients. Little research has been done on hemotoxins, but it is known that their protein components help fight off diseases and create stronger antibodies in animals.
Methodology: Testing Testing and experiments will be performed on large animals (i.e. Horses and Pigs). These have very similar organ systems, relative size, and to some extent relative immunity. Formaldehydewill be addedtothevenombeforeanyinjectiontoleveldownthetoxicitylevel, thenitwill be dilutedwithdistilledwater.
Methodology: Testing Human testing will have three test groups. The control being humans (50) that will have no supplemental boosts. Fifty(50) others will consume immune boosters sold on pharmacies (which are basically a mixture of vitamins and antioxidents) The last fifty(50) will be injected with the snake venom and water solution. These tests will be done in a year and a half period and in the same city to ensure same environmental factors.
Methodology: Testing Testing will take about a year and a half. Due to the fact that increasing anti-bodies, creating a natural defense and raising the immune system can take several months. It takes approximately 8 months for a Horse to create these antibodies and raise its immunity. Testing will be performed with hemotoxins.
Methodology: Process The hyper-immunization will begin with a diluted venom, and then injected directly into a horse and into a pig. After several injections, usually twice a week, blood tests will be performed every other week to check progress. Venom will start with a 15:1 dilution, and work its way up until it reaches a 5:1.
Expected Results Humans injected with the snake venom dilution will show a stronger and more aggressive immune system than the other two. While the ones taking the vitamin supplement will also show an increase in immunity, it will not be as aggressive nor strong as the snake venom.
References What is snake venom?, ThinkQuest, 2010, Oracle ThinkQuest, July 18,2010, http://library.thinkquest.org/C007974/2_6sve.htm Viegas, J., (2010), Cobra Venom Erases Arthritis Symptoms, 1(1),Discovery Channel, July 24,2010, http://news.discovery.com/animals/cobra-venom-arthritis-pain-relief.html R.D.G. Theakston, (1989), Applied immunology in snake venom research, 83(6), Tropical Medicine and Hygiene, July 24, 2010, http://www.journals.elsevierhealth.com/periodicals/trstmh/article/0035-9203(89)90312-X/abstract
References Unknown author, 2010, Snake Venom 2, 1(1) 2010, Immune Clinic Research, July 20, 2010, http://www.immuneclinicresearch.com/4/SNAKE-VENOM-2.html P. Gopalakrishnakone,1994, Sea Snake Toxinology,1(1), Page 128, Venom and ToxinResearchGroup, July 18, 2010, http://books.google.com.pr/books?id=i2yTrfdnnq8C&pg=PA128&lpg=PA128&dq=Snake+poison+immunology&source=bl&ots=mX-aV2mM_p&sig=OuCizaYJfZ6hzxNoYLmSXK7pheA&hl=es&ei=Dbw4TPXuOISdlgfr7IHWBw&sa=X&oi=book_result&ct=result&resnum=9&ved=0CFsQ6AEwCDgK#v=onepage&q&f=false