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The study was done to detect simultaneously the antigen and the antibodies in HIV patients attending the STD-OP of a tertiary care centre. A total of 114 patients were screened for HIV status. The regular protocol by NACO has detected only one case out 114 patients, whereas simultaneous detection of Ag and Ab by ELISA method has detected 2 more patients. This study highlights the necessity for using the newer generation kits in detecting new cases at the earliest and to reduce the window period.