• Carry out an experiment by using carbon dioxide sensor to detect the carbon dioxide release by yeast during fermentation.• Different concentration of glucose is used to observed the different in carbon dioxide concentration collected.• Data logger will collect time data and plot a graph
Discussion1. Based on the table, we can observe that the concentration of carbon dioxide increase from time to time, what leads to this increment? The changes of carbon dioxide level indicated that the fermentation of yeast in glucose has begun because more and more carbon dioxide is release.
2. What is the difference of above respiration with human respiration? The release of carbon dioxide indicates that this is an anaerobic respiration. On the other hand, human respiration is an aerobic respiration. The main differences in anaerobic and aerobic respiration is oxygen is absent in anaerobic respiration while oxygen is present in aerobic respiration. Oxidation of glucose is not complete in anaerobic respiration but it is complete in aerobic respiration.
3. What are the similarities of above respiration to human respiration? Both of the respirations are cellular respiration which involved the breakdown of glucose to release energy that stores in ATP molecules.4. Why paraffin oil is added to cover the glucose solution? Paraffin oil is added to prevent air from dissolving in the glucose solution which might affect the result.
5. What is the function of pressure valve in the experiment? The valve is close to prevent air from atmosphere to enter the conical flask which we are monitoring the carbon dioxide release by fermentation of yeast.6. Write an equation for the above experiment.C6H12O6 + 2 C3H5OH 2CO2 + 210 KJGlucose Ethanol Carbon dioxide Energy
7. Calculate the rate of respiration of each concentration of glucose. Which concentration has the higher respiration rate?rate of respiration for 10% glucose = = 35.45 ppm/minrate of respiration for 5% glucose = = 114.22 ppm/min Based on the calculation, we can observe that 5% glucose has the higher rate of respiration. Calculation we obtained match the graph that we plotted before.
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