Frustrated with Protein Expression?


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Corynex is a novel Protein Expression system that overcomes some of the most frustrating challenges with common microbial expression systems by secreting fully folded and active proteins directly into the cell medium with minimal host proteins and impurities. This greatly simplifies the entire purification process, lowers costs, and ultimately speeds time to market.

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Frustrated with Protein Expression?

  1. 1. Corynex® A New Solution to Protein Expression 1
  2. 2. 2 biologic medicines large molecules made in living organisms to produce proteins which treat diseases Biologics offer treatments for some of the most challenging diseases including cancer, HIV/AIDS, Alzheimer’s disease, and more. By reconstructing segments of DNA, researchers are able to produce the desired proteins (recombinant proteins), which target a specific disease. Worldwide nearly 200 biologic medicines have transformed the lives of over 800 million patients with serious diseases. Protein Expression Systems are how these proteins are produced.
  3. 3. What is a Protein Expression System? Protein Expression Systems refer to the type of living organism that is used to grow the desired protein. 3 The most common expression systems for recombinant biopharmaceuticals are: Each system varies with respect to protein quality, cost, expression amount, and speed.
  4. 4. Microbial Expression Systems Advantages: • Affordable • Easy to Culture • Grow Fast • High Titer Drawbacks: • Low Purification Yield • Misfolded Protein • Aggregation • Protein Degradation • Denaturation • Cost/Time of purification 4
  5. 5. Is there a Solution? 5 Yes! Corynex® is a breakthrough, patented system that overcomes common challenges with conventional microbial expression systems by allowing for: • Isolation of higher yields • Biologically active proteins • Accurate folding • Fewer host proteins • Increased purity • No endotoxins
  6. 6. So what exactly is Corynex? Corynex uses the strand of bacterium, Corynebacterium glutamicum to produce these complex and difficult-to-express proteins Corynebacterium glutamicum: Discovered in Japan (1956) as a producer of amino acids for food production Gram-positive soil bacterium Generally Regarded as Safe (GRAS) Ajinomoto Althea discovered it can express therapeutic proteins  tremendous biopharma impact 6 Non-sporulating, non-pathogenic, non-endotoxic
  7. 7. How does Corynex® work? 7 Promoter Target protein DNA Target protein Transformation Cultivation Recombinant Corynebacterium glutamicum Plasmid vector Signal peptide Corynebacterium glutamicum Secretion Corynex® transforms Corynebacterium glutamicum with DNA plasmids containing the target recombinant gene. This gene is transcribed using RNA polymerase producing an mRNA message. The mRNA message is bound to a ribosome complex and begins translation into an unfolded protein molecule containing a unique peptide signal sequence. These protein molecules begin accumulating in the Corynebacterium glutamicum cytoplasm before they are ready for secretion. The proteins are then secreted though one of 2 pathways: SEC pathway and TAT pathway, and accurately fold directly in the media. Step 1 Step 2 Step 3 Step 4
  8. 8. Corynex Process 8 IGF-1 Fermentation Separation Chromatography IGF-1 Fermentation Separation Lysis Separation Refolding Chromatography IGF-1 production process using E.coli IGF-1 production process using Corynex®
  9. 9. Corynex Track Record (since2009) • 7 out of the Global Top 20 Pharma • Licensed by a Big Pharma company • Mid-size Pharma, Ventures etc. • USA, Europe, Japan, Asia Number of Customers: >50 companies Trial Expression: >150 proteins • Therapeutic proteins, reagent proteins, enzymes etc. Expression success rate: >40% • Impressive considering Corynex is often tried when others have failed. • SEC pathway, TAT pathway, Fusion method • Improved success rate by utilizing all 3 methods Process Development: >10 proteins • Over 10 proteins have gone through entire process development stages 9
  10. 10. So Why Look into Using Corynex? 10 Secretes protein directly into media No need for cell disruption High initial purity Less purification steps Proteins already in active form No need for refolding For our customers  less downstream costs ~50 - 90% of manufacturing costs of biologics related to downstream To learn more contact Althea at