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Cell-mediated immune responses
 

Cell-mediated immune responses

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Allergy fellow review from textbooks and journals for educational purposes only

Allergy fellow review from textbooks and journals for educational purposes only

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    Cell-mediated immune responses Cell-mediated immune responses Presentation Transcript

    • Cell-Mediated immune responses (Basic Immunology) 15/07/52
    • scope -Phases of T Cell Responses -Antigen Recognition and Costimulation -Functional Responses of T Lymphocytes to Antigens and Costimulation -T Cell Signal Attenuation -Migration and retention of effector T cells at site of infection
    • Phases of T Cell Responses
    • Antigen Recognition and Costimulation
    • TCR complex and its restriction
    • Components of TCR complex
    • Ag receptor of γδT cells -TCR γ & δ chain consist of extracellular Ig-like V & C regions, hydrophobic transmembrane segment, short cytoplasmic tail เช่นเดียวกับ TCR αβ But not express CD4/CD8 -มักพบใน epithelial tissue (intraepithelial lymphocyte) -recognition : hypothesis recognize Ag ที่พบบ่อยบน epithelial boundaries between host & external environment
    • Structure of CD4 and CD8 coreptor
    • Costimulatory receptors of CD28 & CD2 families &their ligand
    • Role of costimulators in T cell activation -2nd signal -absense : fail to respond & die by apoptosis or enter state of unresponsiveness (anergy) -increase production of IL-2 & stabilize IL-2 mRNA -promote survival of T cell
    • Role of costimulators in T cell activation 1).CD28 bind to B7-1(CD80), B7-2(CD86) on APC (dendritic cell, macrophage), B cell 2).ICOS(inducible costimulator) : activate production of IL-10 3)CTLA-4(CD152) : self tolerance
    • Others accessory molecule on T cells -CD44 acidic sulfated membrane glycoprotein : mature T cell, thymocyte, B cell, macrophage, granulocyte, erythrocyte, fiboblasts -bind to hyaluronate : retention T cell in extravascular tissue at infection site -binding of activated & memory T cell to endothelium at site of inflammation, in mucosal tissue
    • Others accessory molecule on T cells (con.) -CD40 ligand express บน activated CD4 T cell bind to CD40 บน B cell, macrophage, dendritic cell, endothelial cell -Ligand for Fas (CD95) -apoptosis -important for eliminating T cell that are repeated stimulated by Ag
    • Accessory molecules of T lymphocyte
    • Ligand-receptor pairs involved in T cell activation
    • Regulation of integrin avidity
    • Clonal expansion (proliferation)
    • Signal transduction pathways in T lymphocyte
    • Early tyrosine phosphorylation events in T cell activation
    • Early tyrosine phosphorylation events in T cell activation
    • T cell signaling downstream of PLCγ1
    • Ras-Map kinase pathway in T cell activation
    • Activation of transcription factors in T cell
    • T cell signal attenuation ทำำได้ 3 วิธี 1).activation inhibitory receptor of CD28 2).tyrosine-phospatases (SHP-1) 3).protein known as E3 ubiquitin ligases
    • T cell signal attenuation
    • Ubiquitin ligase Cbl-b in terminating T cell responses
    • Differentiation of naïve T cell into effector cell
    • Development of Th1 and Th2 subset
    • Cytokine signaling by the Jak-STAT pathway
    • Characteristics of subsets of CD4+ T cell
    • Effector function of Th1 cells
    • Effector function of Th2 cells
    • Activation of CD8+ T cells
    • Differentiation of CD8 T cell into CTL -ต้องกำร IL-12 -transcription factor : T-bet & eomesodermin -differentiated CTL secrete cytokine, mostly IFN-γ, lymphotoxin, TNF -cascade in differentiation same as CD4
    • Effector of CD8+ T cell steps in CTL-mediated lysis of target cell
    • Mechanisms of CTL-mediated lysis of target cells
    • Action and functions of macrophages in CMI
    • Migration and retention of effector T cells at site of infection
    • Migration of naïve and effector T lymphocytes
    • Recruitment of leukocyte at infection site
    • T cell homing receptors and their ligands
    • Thank you for your attention