Biosensors, ATP biosensor, IDM7

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Done By: WB_Challengers7
School Name: Al Wakra Independent Secondary School for boys



Biosensors: Students investigate the use of biological molecules as materials and use enzymes as chemical sensors in the design of diagnostic tests for peroxide, cholesterol, and glucose.

we choose this project because we got this idea from Investigating Biological Molecules and Bioluminescence activity. Searching for the ATP with this way will help the scientists to know if there is ATP or no ATP and that way won’t take time as they can do it very fast .

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Biosensors, ATP biosensor, IDM7

  1. 1. AL-BAIRAQ I AM DISCOVERING MATERIALS BIOSENSORS Challengers7 Mohamed El deeb Hamad a altamimi Ahmed h al-abdulgani
  2. 2. OUTLINE • Introduction • Investigating Biological Molecules and Bioluminescence • Investigating enzymes and indicator molecules • Making a peroxide biosensor • Testing a cholesterol biosensor • Design Project (ATP Biosensor) • Product Idea
  3. 3. INTRODUCTION Biosensors What are sensors ? Nose! Tongue! Ears! Eyes! Fingers! Sensors can give qualitative or quantitative analysis.
  4. 4. Sensors can be divided into three types physical sensors chemical sensors biosensors
  5. 5. What is a Biosensors ? Biosensors are which are of providing either qualitative or quantitative results. The biological sensing element has to be connected to a transducer so that a visually observable response occurs. Biosensors function by coupling a biological sensing element with a detector system using a transducer.
  6. 6. Where are Biosensors Being Used ? Healthcare (glucose, artificial pancreas) Food and drink production and analysis Pollution control and monitoring
  7. 7. Objective To see if the whole fireflies, whole firefly tail lanterns and firefly tail lantern pieces will give off light after adding the buffer and ATP Compare each of three firefly samples with the control Notice which samples will give off light
  8. 8. Procedure 1. Label 4 tubes A,B,C and D 1- Two whole fireflies for 1st tube (W) 2- Two whole fireflies tail lanterns for 2nd tube (T) 3- Two firefly tail lantern pieces for 3rd tube (P) 4- last tube for control sample we (C) don’t contain firefly materials Add 2 mL phosphate buffer and 0.1 mL ATP for each tube Test the tubes in darken room and notice which tube will give off light
  9. 9. Results Tube A,B and C will give off light Tube D won’t give off light The purpose of the control is to demonstrate that the firefly materials, not the buffer or the ATP, are responsible for the luminescence
  10. 10. Conclusion The firefly can give off light again if we add buffer and ATP we reactive it again The reason of the luminescence is the firefly materials not the buffer and the ATP The firefly materials give signal if we add buffer and ATP We can use the firefly materials to search for the ATP ( ATP sensor)
  11. 11. summary Firefly give signal when we add phosphate buffer and ATP We can use Firefly materials as ATP sensor firefly tail lantern pieces will give off stronger light than the whole firefly tail lanterns
  12. 12. INVESTIGATING ENZYMES AND INDICATOR MOLECULES Activity (2)
  13. 13. Prepare 3ml of a 1% peroxide solution . . Label 4 tubes (A.B.C.D)and follow the table Prepare 0.1ml peroxidase darken the room . Record all observations. Procedure
  14. 14. Test Tube Peroxide Indicator peroxidase Type of signal A 0.1ml 1% H2O2 Tip-full of luminol peroxidase Light blue B 0.1ml 1% H2O2 Tip-full of sodium luminol peroxidase Light Faint blue C 0.1ml 1% H2O2 0.2ml 4CN solution . peroxidase Purple color D 0.1ml 1% H2O2 0.2ml TMB solution . peroxidase Yellow color Results
  15. 15. Light . Blue signal and we use Tip-full of luminal indicator . Light . Faint blue signal . And we use Tip- full of sodium luminal indicator . Using 4CN solution as indicator . Give us purple color signal . We have yellow color signal . When we use TMB solution as indicator . Conclusion
  16. 16. Activity (3)
  17. 17. Objective
  18. 18. Concentration of peroxide Observation of sensor strip 0.0003% 0cm 0.00075% 0.5cm 0.003% 1cm 0.0075% 2cm 0.025% 2.5cm 0.075 2.75cm 0.3 3cm 1% 3.5cm
  19. 19. The increasing of peroxide concentration , increase the sensor strip observation . The highest cone of peroxide = 1% Given us 3.5 cm sensor strip . Conclusion
  20. 20. TESTING A CHOLESTEROL BIOSENSOR Activity (4)
  21. 21. Set up 6 tubes A-F Set up a control (Tube A and 0.2ml 10% trition ) And the cholesterol sample And the enzyme solution And 2ml TMP indicator solution (to add) Procedure
  22. 22. Test tube Cholesterol cone 1 0.5 F 1:2 0.25 E 1:4 0.125 D 1:8 0.0625 C 1:16 0.3125 B 0 0 A Concentration of the Cholesterol Cholesterol + O2 Cholesterol oxidase Oxidized form of cholesterol + H2O2
  23. 23. Tube (1-F) represent the high concentration of cholesterol Tube (B- represent 1:16 ratio) consider the lowest concentration of cholesterol . Conclusion
  24. 24. ATP BIOSENSOR
  25. 25. Important of ATP it gives the cells energy to perform the tasks it needs to do to keep the body moving. It makes the muscles of the body move. Without it the cells would die and therefore so would the body. ATP is important because the energy that is released, when its phosphate bonds are hydrolyzed, is the energy that is used to drive endergonic reactions in cells.
  26. 26. Objective Designing a Unique ATP Biosensor Designing ATP Biosensors Using Luciferase Enzyme Use the firefly materials and phosphate buffer to design ATP
  27. 27. Procedure Gather these materials 1- 2 tubes and Label them A and B 2- 4 firefly tail lantern pieces 3- 4 mL phosphate buffer 4- 0.1 mL ATP A B 2 firefly tail lantern pieces 2 firefly tail lantern pieces 2 mL phosphate buffer 2 mL phosphate buffer 0.1 mL ATP No ATP Check the tubes and darken room
  28. 28. Results A B luminescence No luminescence
  29. 29. Conclusion We Can make an ATP Biosensor by using the firefly materials and phosphate buffer Tube A give off light because it’s contain ATP Tube B didn’t give off light because it doesn’t contain ATP
  30. 30. INTRODUCTION AND RATIONALE we choose this project because we got this idea from activity (1) Investigating Biological Molecules and Bioluminescence and the ATP is important searching for the ATP with this way will help the scientists to know if there is ATP or no ATP and that way won’t take Time As they can do it very fast and this is new idea and now one think of it before and this will help the scientists in Qatar. objectives : Design an ATP biosensor
  31. 31. we expected that answer of your research question will be that the ATP and the firefly materials will give off light so that will be ATP biosensor according to out experience with Albairaq workshops and the scientific background and the researches related to our project.
  32. 32. OUR PROJECT STORY We got the project idea from al-biraq activities and we work on it to get it better and make new Biosensor . Al-biraq activities were very useful and it helped us to get the idea . We used the internet to get more information about the ATP and how can we get that biosensor better . Our project story start from al-biraq activities and the Biosensor Module
  33. 33. PRODUCT IDEA RESULTS We used our product to search for the ATP and we designed an ATP Biosensor we found out that
  34. 34. RECOMMENDATIONS Using ATP biosensor is very easy and it’s very accuracy If anyone want to do work on this research I advice him to try to get the concentration of the ATP because we couldn’t get it
  35. 35. CONCLUSIONS (OVERALL) - The firefly can give off light again if we add buffer and ATP we reactive it again - The reason of the luminescence is the firefly materials not the buffer and the ATP - We can use the firefly materials to search for the ATP - We Can make an ATP Biosensor by using the firefly materials and phosphate buffer - ATP + buffer + firefly materials == luminescence
  36. 36. ACKNOWLEDGMENT Thank all people, teachers, friends, and your school that supported you during your journey in AL-bairaq project. Also don’t forget to thank Eng. Doaa from AL-Bairaq team and the sponsors who gave you the opportunity to participate in AL- Bairaq project, create and innovate.

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