Prosedur Pembuatan Reagen Untuk Pewarnaan
Upcoming SlideShare
Loading in...5
×
 

Prosedur Pembuatan Reagen Untuk Pewarnaan

on

  • 948 views

Prosedur Pembuatan Reagent Untuk Pewarnaan

Prosedur Pembuatan Reagent Untuk Pewarnaan

Statistics

Views

Total Views
948
Views on SlideShare
871
Embed Views
77

Actions

Likes
1
Downloads
22
Comments
0

1 Embed 77

http://alatalatlaboratorium.com 77

Accessibility

Upload Details

Uploaded via as Microsoft PowerPoint

Usage Rights

© All Rights Reserved

Report content

Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

Cancel
  • Full Name Full Name Comment goes here.
    Are you sure you want to
    Your message goes here
    Processing…
Post Comment
Edit your comment

Prosedur Pembuatan Reagen Untuk Pewarnaan Prosedur Pembuatan Reagen Untuk Pewarnaan Presentation Transcript

  • Prosedur Pembuatan Reagent Untuk Pewarnaan . www.AlatAlatLaboratorium.com/Blog
  • Daftar Isi Acid Fast Stain / Pewarnaan Bakteri Tahan Asam. Capsule Stain / Pewarnaan Kapsul. Fungal Stain / Pewarnaan Jamur. Gram Stain / Pewarnaan Gram.
  • Daftar Isi Negative Stain / Pewarnaan Negatif. Spore Stain / Pewarnaan Spora.
  • Acid-fast Stain
  • Solution A  Basic Fuchsin (90% dye content) = 0,3 g  Ethyl alcohol (95%) = 10,0 ml Solution B  Phenol = 5,0 g  Distilled water = 95,0 ml Note : Mix solutions A and B. Add 2 drops of trition X per 100 ml of stain for use in heatless method
  •   Ethyl alcohol (95%) = 97,0 ml Hydrochloric acid = 3,0 ml
  •   Methylene Blue = 0,3 g Didtilled water = 100,0 ml
  • Capsule Stain
  •   Crystal Violet (85% dye Content) = 1,0 g Distilles Water = 100,0 ml
  •   Copper Sulfate (CuSO₄ . 5H₂O) = 20,0 g Distilled water = 80,0 ml
  • Fungal Stains
  • Lactic acid = 20,0 ml  Phenol = 20,0 g  Glycerol = 40,0 ml  Distilled water = 20,0 ml  Aniline Blue = 0,05 g Note : Heat Gently in hot water (double boiler) to dissolve; then add aniline blue dye 
  •   Gram`s iodine (as in Gram`s stain) = 10,0 ml Distilled water = 30,0 ml
  • Gram Stain
  • Solution A  Crystal Violet (90% dye content) = 2,0 g  Ethyl alcohol (95%) = 20,0 ml Solution B  Ammonium oxalate = 0,8 g  Destilled water = 80,0 ml Note : mix solutions Aand B
  •    Iodine = 1,0 g Potassium iodide = 2,0 g Distilled water = 300,0 ml
  •   Ethyl Alcohol (100%) = 95,0 ml Distilled water = 5,0 ml
  •    Safranin O = 0,25 ml Ethyl alcohol (95%) = 10,0 ml Distilled water = 100,0 ml
  • Negative Stain
  • Nigrosin, water-soluble = 10,0 g  Disrilled water = 100,0 ml Note : Immerse in boiling water bath for 30 minutes.  Formalin = 0,5 ml Note : Filter twice through double filter paper 
  • Spore Stain
  •   Malacite green = 5,0 g Distilled water = 100,0 ml
  •    Safranin O = 0,25 ml Ethyl alcohol (95%) = 10,0 ml Distilled water = 100,0 ml
  • Our Website www.AlatAlatLaboratorium.com. Toko Online Alat Laboratorium www.AlatAlatLaboratorium.com/Blog Panduan Memilih Alat Laboratorium www.AlatAlatLaboratorium.com/LaboratoriumMikrobiologi Daftar Peralatan Pembuatan Laboratorium Mikrobiologi www.AlatAlatLaboratorium.com/Memmert Informasi Product Memmert di Indonesia
  • Facebook : AlatAlatLaboratorium. Q+A Twitter : Alat2Lab. Telp/Sms : 0852-1304-3138. E-mail : sales@AlatAlatLaboratorium.com.
  • THANK YOU!