Tissue culture
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Tissue culture

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TIssue culture

TIssue culture

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  • 1. Tissue Culture Fun Fruit Nutrition The Guide Feb – 2012 Biotechnology By: Afida Zahara Adzkiya Alifah Farah Gusmaniar Indah Puspita Aziz Ridha Astri Indryana1987 Street Ave City, State Balanced Nutrition Coming to Fruition www.funFruit.com 60623, US.
  • 2. FunFruitNutrition Definition of tissue culture Tissue culture is anactivity to cultivate a tissue of plantvegetatively to be a plant that hassimilar property to its parent in ashort time. Plants can be multipliedusing this technique as plants havea totipotency ability, meaning thatfrom a tissue they can grow anddevelop into new individuals.Tissue culture is used to solve foodshortage problems. 1987 Street Ave City, State Balanced Nutrition Coming to Fruition www.funFruit.com 60623, US.
  • 3. FunFruit Information Layout Slide with TableNutrition Tissue culture was found and well known by F.C Steward, a plant physiologist from United States. Who did his research in 1989. Steward try to make tissue culture of carrot and he is success. Tissue culture well because the work of Muer, Hildebrant, and Riker in 1954. 1987 Street Ave City, State Balanced Nutrition Coming to Fruition www.funFruit.com 60623, US.
  • 4. FunFruit The Various Uses of Tissue CultureNutrition• Obtaining a lot of germs in a short time with similar property to the parent.• Making plants with properties we want.• Tissue culture is economical from aspect of time , space, and energy.• Produce plants free from virus and disease. 1987 Street Ave City, State Balanced Nutrition Coming to Fruition www.funFruit.com 60623, US.
  • 5. The Procedure of Tissue Culture Technique Cultivating tissue in Preparing growth Preparing tissue liquid growth medium medium Moving plant whoseCultivating plant in Cultivating plant in root bud and leaf bud actual land pot containing soil have grown to another solid growth medium 1987 Street Ave City, State Balanced Nutrition Coming to Fruition www.funFruit.com 60623, US.
  • 6. 1 Preparing growth medium. Substance that must exist in growth medium consist of minerals (macro element, such as C, H, O, N and micro element such as Zn, Mo), amino acid, vitamin, sugar, and hormone in certain amount.1987 Street Ave City, State Balanced Nutrition Coming to Fruition www.funFruit.com 60623, US.
  • 7. 2 Preparing tissue. The tip part of a young shoot, such as root and leaf tips, are cut into 1-1.5 mm³. Next, the tissue is sterilized using 5% hypochlorite calcium and is washed off several times with sterile aquadest.1987 Street Ave City, State Balanced Nutrition Coming to Fruition www.funFruit.com 60623, US.
  • 8. 3 Cultivating tissue (explants) in liquid growth medium. Explants are placed in a mixer table (shaker) that shakes at 60- 70 times per minute. The mixing is performed for 6 hours a day for 1.5 – 2 months . Shaking is meant for faster distribution of substance and food absorption as well as process of gas exchange. In about 2 months, callus will grow in this liquid medium.1987 Street Ave City, State Balanced Nutrition Coming to Fruition www.funFruit.com 60623, US.
  • 9. 4 Moving plant whose root bud and leaf bud have grown to another solid growth medium.1987 Street Ave City, State Balanced Nutrition Coming to Fruition www.funFruit.com 60623, US.
  • 10. 5 Cultivating plant in pot containing soil. The plants whose rot bud and leaf bud have grown are removed to another solid medium to be separated, so bigger plants can grow.1987 Street Ave City, State Balanced Nutrition Coming to Fruition www.funFruit.com 60623, US.
  • 11. 6 Cultivating plant in actual land. Before being cultivated in actual land, the plant from solid medium is moved or cultivated first in a pot that contains soil that has been given fertilizer. If the condition of plant has been strong, it can be moved to actual land.1987 Street Ave City, State Balanced Nutrition Coming to Fruition www.funFruit.com 60623, US.
  • 12. Apakah objek yang dikultur oleh pempopuler tekhnik kultur jaringan (Muer, Hildebrant, and Riker) sama dengan objek yang dikultur oleh F. C. Steward?Tidak sama.Hildebrant berhasil mengembangkan tekhnik kultur jaringan pada sel-sel rambut darijaringan mesofil daun tanaman monokotil.Muer berhasil menumbuhkan tanaman lengkap dari kultur sel tunggal.Kalau Riker kami belum mengetahui apa objek yang dikulturnya, namun padalogikanya itu pasti berbeda dengan objek yang dikultur oleh F. C. Steward. 1987 Street Ave City, State Balanced Nutrition Coming to Fruition www.funFruit.com 60623, US.
  • 13. Question from Sabila: “What kind of plant that usually used for tissue culture?”Pada dasarnya semua jenid tumbuhan dapat dikultur, karena semua tumbuhanmemiliki sifat totipotensi di jaringan meristem (ujung akar dan batang). Namuntumbuhan yang biasanya dikultur adalah:-Yang pertumbuhannya lambat atau susah-Memiliki nilai jual yang tinggi-Banyak bergunan bagi masyarakat-Contohnya adalah tanaman anggrek, jati dan tebu. 1987 Street Ave City, State Balanced Nutrition Coming to Fruition www.funFruit.com 60623, US.