Development of breeding lines using marker technologies: The approach used by the Genetics and Genomics Platform for Rice ...
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Mars poster very last version

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Mars poster very last version

  1. 1. Development of breeding lines using marker technologies: The approach used by the Genetics and Genomics Platform for Rice in Africa FROM Maker-Assisted Selection (MAS) TO Marker-Assisted Recurrent Selection (MARS) TOWARD Genome-Wide Marker-Assisted Selection (GWMAS) Selection of parental lines (P1 and P2) Inbred 1 x Inbred 2 Extraction of evaluation F2 derived-lines (BC, 3 NARS Crossing : P1 (recurrent parent) x P2 (donor parent) F2 for genotyped plants ways cross, double cross) Breeding value : Estimation F1 F3:4 of marker effect X P1 Genotyping Plant selection based on best GEBV* BC1F1 QTL estimation Cycle 1 Selection of Np plants (144) , content and Foreground selection Identification of BC1F1 individuals that are heterozygous for the closest marker and If large population size F3:5 High density genotyping Selection of 4 plants homozygous at the flanking markers Genetics and (128-256 markers) and Inter-mating effect Backcross of the selected BC1F1 individuals with P1 to produce BC2F1 BC2F1 Genomics Phenotyping Recombination Platform for Whole genome background selection Choice of 24 seeds Identification of BC2F1 individuals that areheterozygous for the closest marker with the Rice in Africahighest proportion of the recurrent parent genome Selection of lines based on genetic per crossusing higher molecular marker number. Self the selected individuals to produce BC2F2 value, molecular score Cycle 2 Selection of 144 plants weight, intensity of selection… BC3F1 Genotyping , selection of 4 Genotyping and genetic gain simulation in crossing selected lines plants and inter-mating (Optimas: GCP IBP tool) 2x Recombination Inter-mating of selected lines Choice of 24 seeds BC3F3 per cross Genotyping and genetic gain simulation in crossing selected lines (Optimas: GCP IBP tool) Selection of 144 plants Inter-mating of selected lines Improved lines Repeat the procedure Advanced for 2 cycles Research Extract fixed lines Institutes Evaluation and advanced testing in conventional breeding * Genomic Estimated Breeding Values
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