Talinum paniculatum

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  • 1. TALINUM PANICULATUM (GINSENG JAWA)
    20 DAYS
  • 2. Group members…
    AMIRAH BINTI SHAARI
    HILDA ANAK EMAK
    AIN NAJWA BINTI AMRAN
    NURUL HIDAYAH BINTI MANSOR
    ONG SHWU CHYN
    PUI WAN TING
    WONG SIEW CHING
    NATASHA KAUR
    AMARPREET KAUR
    YAASHINI SELVARAJAH
    KRISNAVIMALA KRISHUAN
    NABILAH BINTI YUSOF
    NORIZA BINTI ISHAK
  • 3. INTRODUCTION
  • 4. FiveMusmusculushave been used to investigate the effect of TalinumPaniculatum(ginseng jawa)
    to the liver, kidney and intestine within `
    20 days of experimental.
  • 5. Problem statement
    Why do we choose
    Musmusculusas our specimen?
    • Class of mammalian
    • 6. Easy to get
    • 7. Easy to handle-small in size
  • Purpose of the study
    To know the effect of TalinumPaniculatum
    size and
    body weight of Musmusculus
    Behaviour investigation of the
    Musmusculus.
  • 8. Significance of study
    TalinumPaniculatumcontains
    vitamin c and
    toxic
    Tanin,
    Saponin and
    Flavoid.
    So we carry out this experiment to see the side effects of TanilumPaniculatum to the internal organs (intestine, kidney and liver) of Musmusculus.
  • 9. Research limitation
    Experiment applicable only to five Musmusculus.
    only three leaves of TanilumPaniculatum used everyday.
    Time limitation to only 20 days
  • 10. LITERATURE REVIEW
  • 11. 1.Talinum:A Summer Vegetable For Florida
    DAVID FAIRCHILD,
    COCONUT GROOVE,
    FLORIDA
  • 12. DR J.L HEID said that in his report :
    In the green leaves ofTalinum
    The Vitamin C
    is about 160 mg per 100 gram
    The Talinumshow the phenomenally high content of vitamin C compared to guavas and sweet peppers.
    It also probably has a considerable contents of carotenoid pigments.
  • 13. 2The Effect Of Ginseng Jawa Root (TalinumPaniculatum) To The White Male MencitEviSetiyaManuputyFaculty Of Pharmacy University Of Muhammadiyah Surakarta
  • 14. Chemical content in Talinumpaniculatumcomposed of
    saponin,
    flavanoid and
    tanin.
    Saponin - strengthen our body and
    - improve blood circulation.
    Saponin : can cause irritation in mucosa membrane, and can damage red blood cell through hemolysis
  • 15. The root can be used to treat :
    tiredness,
    dizzy,
    diarrhea,
    aphrodisiacs and
    pneumonia.
    The leaves can be used to treat bump.
    Also increase the sexual desire in male mencit.
  • 16. Materials and methods
  • 17. 20 days of observation
    5 mice (Musmusculus)
    Bedding
    3 ginseng jawa (Talinumpaniculatum) leaves (0.5g) mix with 100 ml water
    Mice food
    Mice is weighed daily
    Amount of water consumed are measured
  • 18. Dissection
    Glove
    Cotton and chloroform
    Dissecting set
    Dissecting tray and pins
    Water
    Beaker
    Water was applied on the ventral side of mice
    The kidney, small intestine and liver were dissected.
  • 19. Stages/technique in preparing slide
    Fixation.
    Dehydration.
    Clearing.
    Embedding.
    Sectioning.
    Coloring/ staining.
  • 20. Fixation
    Bouin’s fluid (<24 hours)
    Bottles
    Dehydration
    Ethanol 70% (< 24 hours)
    Ethanol 80%, 95%, 100% (< 1 hour)
    Clearing
    Toluene solution (< 24 hours)
  • 21. Embedding
    (Paraffin processing)
    Paraffin solution (60C) (< 1 hour)
    Paraffin solution (60C) (< 1 hour)
    (twice procedure)
    Paraffin solution added in mold followed by the organ.
    Tissue sample then was left for 5 days.
    • The infiltrating medium (paraffin) provides cellular support for cutting tissue sections.
  • Sectioning (Microtome /cutting)
    materials
    methods
    Diamond pen
    Distilled water
    Albumin
    Slide and slide cover
    Microtome
    Brush
    The slices was placed on the slide.
    Few drops of distilled water/albumin are dropped on the slides.
    Intestine-distilled water
    Albumin - liver and kidney
    Heating (30C)
  • 22. Coloring/staining
    Methods
    Materials
    Color stain used :
    • Harris Hematoksilin- Eosin coloring.
    • 23. Soaking of slides-
    • 24. into 21 piece of jar containing different types of chemicals and liquid
    • 25. based on the duration given.
    • 26. Gluing: DPX / Canada balsam
    • 27. Heating of slides
    • 28. (37 C)
    • 29. 1 week
    Xylen 1 And 2
    Alcohol+xylen (1:1)
    Alcohol, Alcohol( 90%,80%,75%)
    Distilled Water
    Hematoksilin Solution
    0.5 % Sodium Bicarbonate Solution
    Running Water
    Scott’s Solution
    Eosin
  • 30. Results & discussion
  • 31. Graph showing the increasing weight mass of the mice in 20 days
  • 32. The weight of each mice
    increase day by day
    After taking Talinumpaniculatum as their drink.
    The Talinumpaniculatum
    rich with vitamin C
    increases appetite
    Gaining of body weight
  • 33. Graph showing the amount of water and Talinumpaniculatumconsumed by the mice's
  • 34. Intestine (duodenum)
    Affected intestine
    40x magnification
    Goblet cell
    The goblet cell in this intestine secretes more mucous compared to the normal intestine
  • 35. LIVER
    Affected liver
    Normal slide
    40 x magnification
    sinusoid
    Wide spaces in between cells showing the sinusoid. This means that more enzymes are secreted to detoxify the drug (ginseng jawa). In the normal slide the sinusoid is compact.
  • 36. The presence of vitamin C in Talinumpaniculatum causes more enzyme to be released to detoxify the drugs in the liver cells.
    • prove: white spaces (sinusoid)seen in effected liver cells while the original cell the (sinusoid) is more compact.
  • KIDNEY
    Affected kidney
    Normal kidney
    40x magnification
    glomerulus
    The glomerular is tightly packed due to the toxic caused by the drug that is being detoxify by the glomerular as compared to the normal kidney slide.
  • 37. Materials & function:
  • 38. Weight gain
    they will get attracted to mate
    becomes active and aggressive
  • 39. Tannins
    precipitate proteins
    inhibits in some ruminant animals
    for the absorption of nutrients from high-tannin grains
    such as sorghum.
    Tannic acid does not affect absorption
    of other trace minerals such as zinc, copper, and manganese in rats
    • Prove: the glomerulus at kidney cell is tightly packed compared to the original slide of kidney where there are some spaces between the glomerulus.
    The lethal dose ofTalinumpaniculatum around 6% is able to increase the animal's body weight.
    • Prove: the mice’s body weight increases drastically in that 20 days.
  • Problem about the project
    Damage of the weighing machine.
    Damage of Microscope.
  • 40. The end..
    Thank you..