Group members… AMIRAH BINTI SHAARI HILDA ANAK EMAK AIN NAJWA BINTI AMRAN NURUL HIDAYAH BINTI MANSOR ONG SHWU CHYN PUI WAN TING WONG SIEW CHING NATASHA KAUR AMARPREET KAUR YAASHINI SELVARAJAH KRISNAVIMALA KRISHUAN NABILAH BINTI YUSOF NORIZA BINTI ISHAK
FiveMusmusculushave been used to investigate the effect of TalinumPaniculatum(ginseng jawa) to the liver, kidney and intestine within ` 20 days of experimental.
Problem statement Why do we choose Musmusculusas our specimen?
Class of mammalian
Easy to get
Easy to handle-small in size
Purpose of the study To know the effect of TalinumPaniculatum size and body weight of Musmusculus Behaviour investigation of the Musmusculus.
Significance of study TalinumPaniculatumcontains vitamin c and toxic Tanin, Saponin and Flavoid. So we carry out this experiment to see the side effects of TanilumPaniculatum to the internal organs (intestine, kidney and liver) of Musmusculus.
Research limitation Experiment applicable only to five Musmusculus. only three leaves of TanilumPaniculatum used everyday. Time limitation to only 20 days
1.Talinum:A Summer Vegetable For Florida DAVID FAIRCHILD, COCONUT GROOVE, FLORIDA
DR J.L HEID said that in his report : In the green leaves ofTalinum The Vitamin C is about 160 mg per 100 gram The Talinumshow the phenomenally high content of vitamin C compared to guavas and sweet peppers. It also probably has a considerable contents of carotenoid pigments.
2The Effect Of Ginseng Jawa Root (TalinumPaniculatum) To The White Male MencitEviSetiyaManuputyFaculty Of Pharmacy University Of Muhammadiyah Surakarta
Chemical content in Talinumpaniculatumcomposed of saponin, flavanoid and tanin. Saponin - strengthen our body and - improve blood circulation. Saponin : can cause irritation in mucosa membrane, and can damage red blood cell through hemolysis
The root can be used to treat : tiredness, dizzy, diarrhea, aphrodisiacs and pneumonia. The leaves can be used to treat bump. Also increase the sexual desire in male mencit.
Materials and methods
20 days of observation 5 mice (Musmusculus) Bedding 3 ginseng jawa (Talinumpaniculatum) leaves (0.5g) mix with 100 ml water Mice food Mice is weighed daily Amount of water consumed are measured
Dissection Glove Cotton and chloroform Dissecting set Dissecting tray and pins Water Beaker Water was applied on the ventral side of mice The kidney, small intestine and liver were dissected.
Embedding (Paraffin processing) Paraffin solution (60C) (< 1 hour) Paraffin solution (60C) (< 1 hour) (twice procedure) Paraffin solution added in mold followed by the organ. Tissue sample then was left for 5 days.
The infiltrating medium (paraffin) provides cellular support for cutting tissue sections.
Sectioning (Microtome /cutting) materials methods Diamond pen Distilled water Albumin Slide and slide cover Microtome Brush The slices was placed on the slide. Few drops of distilled water/albumin are dropped on the slides. Intestine-distilled water Albumin - liver and kidney Heating (30C)
Coloring/staining Methods Materials Color stain used :
Harris Hematoksilin- Eosin coloring.
Soaking of slides-
into 21 piece of jar containing different types of chemicals and liquid
based on the duration given.
Gluing: DPX / Canada balsam
Heating of slides
Xylen 1 And 2 Alcohol+xylen (1:1) Alcohol, Alcohol( 90%,80%,75%) Distilled Water Hematoksilin Solution 0.5 % Sodium Bicarbonate Solution Running Water Scott’s Solution Eosin
Results & discussion
Graph showing the increasing weight mass of the mice in 20 days
The weight of each mice increase day by day After taking Talinumpaniculatum as their drink. The Talinumpaniculatum rich with vitamin C increases appetite Gaining of body weight
Graph showing the amount of water and Talinumpaniculatumconsumed by the mice's
Intestine (duodenum) Affected intestine 40x magnification Goblet cell The goblet cell in this intestine secretes more mucous compared to the normal intestine
LIVER Affected liver Normal slide 40 x magnification sinusoid Wide spaces in between cells showing the sinusoid. This means that more enzymes are secreted to detoxify the drug (ginseng jawa). In the normal slide the sinusoid is compact.
The presence of vitamin C in Talinumpaniculatum causes more enzyme to be released to detoxify the drugs in the liver cells.
prove: white spaces (sinusoid)seen in effected liver cells while the original cell the (sinusoid) is more compact.
KIDNEY Affected kidney Normal kidney 40x magnification glomerulus The glomerular is tightly packed due to the toxic caused by the drug that is being detoxify by the glomerular as compared to the normal kidney slide.
Materials & function:
Weight gain they will get attracted to mate becomes active and aggressive
Tannins precipitate proteins inhibits in some ruminant animals for the absorption of nutrients from high-tannin grains such as sorghum. Tannic acid does not affect absorption of other trace minerals such as zinc, copper, and manganese in rats
Prove: the glomerulus at kidney cell is tightly packed compared to the original slide of kidney where there are some spaces between the glomerulus.
The lethal dose ofTalinumpaniculatum around 6% is able to increase the animal's body weight.
Prove: the mice’s body weight increases drastically in that 20 days.
Problem about the project Damage of the weighing machine. Damage of Microscope.