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3M Food Safety | Molecular Detection System Technology Sell Sheet
3M Food Safety | Molecular Detection System Technology Sell Sheet
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3M Food Safety | Molecular Detection System Technology Sell Sheet

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3M innovatively combines unique technologies—Isothermal DNA Amplification and Bioluminescence Detection—to offer the specificity and sensitivity you want in a pathogen test solution that is also fast, …

3M innovatively combines unique technologies—Isothermal DNA Amplification and Bioluminescence Detection—to offer the specificity and sensitivity you want in a pathogen test solution that is also fast, simple and cost-effective.

The 3M™ Molecular Detection System uses multiple primers to recognize distinct regions of the genome and Bst DNA polymerase to provide continuous and rapid amplification of genetic material. Pyrophosphate ions (PPi), a by-product of the targeted DNA amplification reaction, and APS, are enzymatically converted into ATP by ATP-Sulfurylase. ATP reacts with luciferase to produce light which is detected indicating the presence of target organism DNA. Both amplification and detection occur simultaneously and continuously during the exponential phase providing real time results and a short run time.

3M Food Safety products are at work in more than 60 countries around the globe. Our passion is work with food and beverage processors to discover new food safety solutions that protect their brand, mitigate their risk and improve their operational efficiencies. With more than 25 years of global experience, we offer a full line of products that work together for consistent, reliable results

For more information, click here http://bit.ly/3M_MDS

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  • 1. 3M™ Molecular Detection SystemA Better Technology3M innovatively combines unique technologies—Isothermal DNA Amplification and Bioluminescence Detection—to offer thespecificity and sensitivity you want in a pathogen test solution that is also fast, simple and cost-effective. Chemistry Overview Isothermal DNA Amplification • Multiple primers recognize distinct regions of the genome • A DNA polymerase with strand displacement activity • Efficient, rapid and continuous amplification of target DNA Bioluminescence Detection • Exponential generation of pyrophosphate, a by-product of the DNA amplification • Pyrophosphate conversion to Adenosine Tri Phosphate (ATP) • Thermostable luciferase uses ATP to generate light Bst DNA Polymerase PPi ATP Light Light PPi ATP- ATP Luciferase Sulfurylase PPi APS Light Light PPi ATP ATPHow does it work?The 3M™ Molecular Detection System uses multiple primers to recognize distinct regions of the genome and Bst DNA polymerase to providecontinuous and rapid amplification of genetic material. Pyrophosphate ions (PPi), a by-product of the targeted DNA amplification reaction, and APS,are enzymatically converted into ATP by ATP-Sulfurylase. ATP reacts with luciferase to produce light which is detected indicating the presence oftarget organism DNA. Both amplification and detection occur simultaneously and continuously during the exponential phase providing real timeresults and a short run time.
  • 2. Technology Comparisons 3M Isothermal DNA PCR (Polymerase 3M Isothermal DNA Immunoassay Amplification Chain Reaction) Amplification Enzyme Bst DNA Polymerase Taq DNA Polymerase Target Nucleic acid (DNA) Protein DNA Exponential amplification Series of antibody/antigen Strand Displacement Heat Reaction Denaturation of target genetic material binding steps Thermal cycling consisting of cycles of repeated heating and cooling of Colorimetric or Reaction Detection Bioluminescence Isothermal (60°C) the reaction for DNA denaturation fluorescence Temperature (94°C) and enzymatic DNA replication (55°C then 72°C) Manual, semi-automated Instrumentation Automated and automated Amplification Continuous Cycling Results Human interpretation Automated Detection Bioluminescent Light Fluorescent Light Interpretation (visual) or automatedA Streamlined SolutionAccurate Fast and Simple• Multiple, specific primers target distinct regions of the genome resulting • The continuous DNA strand displacement process enables efficient and in more efficient amplification of target DNA and accurate results rapid amplification of DNA• Bst DNA polymerase is less susceptible to inhibiting substances found in • Bioluminescence provides real-time detection of the DNA amplification food samples than other polymerases AND simultaneous amplification and detection allows for detection of• DNA is amplified continuously under isothermal conditions limiting the positive results before the end of the run possible interference of the template or DNA polymerase by inhibitors • The DNA polymerase is robust and tolerant to low-quality DNA allowing found in food samples a simple sample preparation and an assay set up with fewer steps• Detection occurs during the exponential phase of amplification • Bioluminescence has a better signal-to-noise ratio than fluorescence enhancing specificity and is not affected by interference from other fluorophores making the• Bioluminescence detection has a better signal-to-noise ratio than process more robust and allowing the same procedure to be followed fluorescence and is not affected by interference from fluorophores that for all assays can be present in some foods (ex. food dyes, acriflavin in enrichment • Because the DNA amplification is detected via bioluminescence, the media, etc.) 3M Molecular Detection System offers the unique use of color-coded assay tubes to differentiate pathogen assays Cost Effective • Isothermal DNA amplification proceeds at a constant temperature, removing the need for complicated instrumentation • Bioluminescence detection eliminates the need for high-cost excitation sources, fluorophores, fluorescent filters and detectors resulting in a robust instrument with minimal maintenance requirementsFood Safety 3M Canada3M Center Post Office Box 5757Building 275-5W-05 London, Ontario N6A 4T1St. Paul, MN 55144-1000 CanadaUSA 1-800-364-3577 Please recycle. Printed in U.S.A.1-800-328-6553 © 3M 2011. All rights reserved. 3M is a trademark of 3M.www.3M.com/3MMolecularDetectionSystem 70-2009-9491-4 Used under license in Canada.

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