• Share
  • Email
  • Embed
  • Like
  • Save
  • Private Content
Gel electrophoresis
 

Gel electrophoresis

on

  • 1,353 views

 

Statistics

Views

Total Views
1,353
Views on SlideShare
1,124
Embed Views
229

Actions

Likes
0
Downloads
52
Comments
0

2 Embeds 229

http://bitsofbiology.blogspot.com 215
http://www.bitsofbiology.blogspot.com 14

Accessibility

Categories

Upload Details

Uploaded via as Microsoft PowerPoint

Usage Rights

© All Rights Reserved

Report content

Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

Cancel
  • Full Name Full Name Comment goes here.
    Are you sure you want to
    Your message goes here
    Processing…
Post Comment
Edit your comment

    Gel electrophoresis Gel electrophoresis Presentation Transcript

    • GEL ELECTROPHORESIS JESSICA PARTIN
    • WHAT IS GEL ELECTROPHORESIS?o A method used in biology to separate DNA strands to create a DNA fingerprint o Separates the DNA by size lengths o Uses an electric current to separate DNA FINAL PRODUCT 
    • STEP 1 Extract DNA from organism and place in tubes
    • STEP 2 Polymerase Chain Reaction *this is used to make millions of copies of the DNA(this is before the process of gel electrophoresis)
    • PCR STEPS Denature= rips apart DNA  Lasts about 1 minute  At about 94˚F Annealing= starts the coping DNA  Lasts about 45 seconds  At about 54˚F Extension= finishes copying DNA  Lasts about 3-5 minutes  At about 72˚F Repeat 30-40 times
    • STEP 3 Place restriction enzymes in DNA(This cuts the DNA into different lengths)
    • STEP 4Dye the DNA andplace it into gel(the gel is usually agarose gel)
    • STEP 5Run electrical current though gel (negative to positive)
    • STEP 6Place in stain, then under UV lighting. The smallest DNA fragments move the fastest and farthest
    • USES Evidence in criminal cases To determine paternity To diagnose genetic diseases Help to determine kinship in animals Compare similarities and differences between species
    • PROS VS. CONSSmall amount of Hazardous starting material material (DNA) needed ExpensiveDNA can be detected regardless of size of Very time consuming DNA*The Macgyver Project used gel electrophoresis
    • FUNNIES;)
    • GO TO THIS WEBSITE: This is a very helpful interactive site that I got most of my information from. If you would like to know more about gel electrophoresis in more detail, I highly suggest this site!learn.genetics.utah.edu/content/labs/gel