• Share
  • Email
  • Embed
  • Like
  • Save
  • Private Content
Gel Electrophoresis
 

Gel Electrophoresis

on

  • 883 views

 

Statistics

Views

Total Views
883
Views on SlideShare
883
Embed Views
0

Actions

Likes
0
Downloads
27
Comments
0

0 Embeds 0

No embeds

Accessibility

Categories

Upload Details

Uploaded via as Microsoft PowerPoint

Usage Rights

© All Rights Reserved

Report content

Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

Cancel
  • Full Name Full Name Comment goes here.
    Are you sure you want to
    Your message goes here
    Processing…
Post Comment
Edit your comment

    Gel Electrophoresis Gel Electrophoresis Presentation Transcript

    • GEL ELECTROPHORESIS By: Darcy Fahey
    • What is Gel Electrophoresis? This technique is used to detect or isolate molecules of a particular size from a mixture of nucleic acids or protein. Process for separating DNA fragments Type of DNA fingerprinting www.wisegeek.com/what-is-gel-electrophoresis.htm
    • What has it been used for? Tracking criminals. Preventing future diseases or hereditary diseases. Providing paternity/ species information
    • Procedure Chop DNA fragments close to the Variable Number Tandem Repeat (VNTR) regions. Put into gel block (agarose) Heated on paper to separate into two strands Complementary probes have a radioactive phosphorous isotope and are paired Placed on X-Ray film
    • Pros Vs. ConsPRO CON DNA can be  Time Consuming detected  Expensive regardless of  Dangerous size  Civil rights Only a small amount of starting DNA needed
    • History 1930s – first report of sucrose for gel electrophoresis discovered 1959 –accurate control of parameters such as pore size and stability (Raymond and Weintraub) 1969 – introduction of denaturing agents especially SDS separation of protein subunit (Weber and Osborn) 1970s – agarose gels 1983 – pulsed field gel electrophoresis enables separation of large DNA molecules 1983 – introduction of capillary electrophoresis (vein)
    • Cool Sites  I read through a really long article , but it was extremely helpful! http://www.ias.ac.in/sadhana/Pdf2009Aug/57 3.pdf This one is much shorter! http://arbl.cvmbs.colostate.edu/hbooks/gene tics/biotech/gels/principles.html