Gel Electrophoresis

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Gel Electrophoresis

  1. 1. GEL ELECTROPHORESIS By: Darcy Fahey
  2. 2. What is Gel Electrophoresis? This technique is used to detect or isolate molecules of a particular size from a mixture of nucleic acids or protein. Process for separating DNA fragments Type of DNA fingerprinting www.wisegeek.com/what-is-gel-electrophoresis.htm
  3. 3. What has it been used for? Tracking criminals. Preventing future diseases or hereditary diseases. Providing paternity/ species information
  4. 4. Procedure Chop DNA fragments close to the Variable Number Tandem Repeat (VNTR) regions. Put into gel block (agarose) Heated on paper to separate into two strands Complementary probes have a radioactive phosphorous isotope and are paired Placed on X-Ray film
  5. 5. Pros Vs. ConsPRO CON DNA can be  Time Consuming detected  Expensive regardless of  Dangerous size  Civil rights Only a small amount of starting DNA needed
  6. 6. History 1930s – first report of sucrose for gel electrophoresis discovered 1959 –accurate control of parameters such as pore size and stability (Raymond and Weintraub) 1969 – introduction of denaturing agents especially SDS separation of protein subunit (Weber and Osborn) 1970s – agarose gels 1983 – pulsed field gel electrophoresis enables separation of large DNA molecules 1983 – introduction of capillary electrophoresis (vein)
  7. 7. Cool Sites  I read through a really long article , but it was extremely helpful! http://www.ias.ac.in/sadhana/Pdf2009Aug/57 3.pdf This one is much shorter! http://arbl.cvmbs.colostate.edu/hbooks/gene tics/biotech/gels/principles.html

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